TY - GEN
T1 - Application of biologically functionalized chromatography to simulate the interaction between MAO and substrates
AU - Shanshan, Wang
AU - Peixiang, Ma
AU - Feng, Qu
AU - Yulin, Deng
PY - 2007
Y1 - 2007
N2 - Monoamine oxidase (MAO) immobilized in the immobilized artificial membrane (IAM) was packed into a capillary to make a chromatography column to simulate the MAO function of catalyzing the oxidative deamination of amine in organism. MAO marked with Quantumdots (QDs) was used as probe to demonstrated that MAO was immobilized in the IAM hydrophobic interface, and the enzymatic activity analysis proved that the immobilized MAO activity remained. The column packed with MAO immobilized in IAM mimic the MAO biological function to DA. These results were hopefully to lead to a new area for chromatography to develop in the study of biological function. A new concept of Biofunctionalized Chromatography was presented in this article, which has a prosperous future in the study of interaction between proteins and small molecules.
AB - Monoamine oxidase (MAO) immobilized in the immobilized artificial membrane (IAM) was packed into a capillary to make a chromatography column to simulate the MAO function of catalyzing the oxidative deamination of amine in organism. MAO marked with Quantumdots (QDs) was used as probe to demonstrated that MAO was immobilized in the IAM hydrophobic interface, and the enzymatic activity analysis proved that the immobilized MAO activity remained. The column packed with MAO immobilized in IAM mimic the MAO biological function to DA. These results were hopefully to lead to a new area for chromatography to develop in the study of biological function. A new concept of Biofunctionalized Chromatography was presented in this article, which has a prosperous future in the study of interaction between proteins and small molecules.
UR - https://www.scopus.com/pages/publications/48149115471
U2 - 10.1109/ICCME.2007.4382057
DO - 10.1109/ICCME.2007.4382057
M3 - Conference contribution
AN - SCOPUS:48149115471
SN - 1424410789
SN - 9781424410781
T3 - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
SP - 1796
EP - 1802
BT - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
T2 - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
Y2 - 23 May 2007 through 27 May 2007
ER -