荧光假单胞杆菌磷脂酶B界面催化分子机制初探

Yiwei Zhao; Fangyan Jiang; Chun Li; Dazhang Dai

doi:10.11949/0438-1157.20200527

荧光假单胞杆菌磷脂酶B界面催化分子机制初探

Yiwei Zhao
, Fangyan Jiang
, Chun Li
, Dazhang Dai^*

^*此作品的通讯作者

化学与化工学院

Beijing Institute of Technology

科研成果: 期刊稿件 › 文章 › 同行评审

1 引用（Scopus）

摘要

The physicochemical property and secondary structure of Pseudomonas fluorescens phospholipase B(Pf-PLB) were analyzed base on its primary structure, and the tertiary structure of Pf-PLB was predicated. A "lid-in, side-out" hypothesis model was put forward to illuminate the possible interfacial catalytic mechanism of Pf-PLB. The structure of Pf-PLB was modified by site-directed mutagenesis, and the proposed mechanism model was verified by combining with the catalytic properties of mutant Pf-PLB.

投稿的翻译标题	Interfacial catalytic mechanism of Pseudomonas fluorescens phospholipase B
源语言	繁体中文
页（从-至）	4255-4259
页数	5
期刊	Huagong Xuebao/CIESC Journal
卷	71
期	9
DOI	https://doi.org/10.11949/0438-1157.20200527
出版状态	已出版 - 1 9月 2020

关键词

Catalytic mechanism
Phospholipase B
Structure prediction

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10.11949/0438-1157.20200527

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title = "荧光假单胞杆菌磷脂酶B界面催化分子机制初探",

abstract = "The physicochemical property and secondary structure of Pseudomonas fluorescens phospholipase B(Pf-PLB) were analyzed base on its primary structure, and the tertiary structure of Pf-PLB was predicated. A {"}lid-in, side-out{"} hypothesis model was put forward to illuminate the possible interfacial catalytic mechanism of Pf-PLB. The structure of Pf-PLB was modified by site-directed mutagenesis, and the proposed mechanism model was verified by combining with the catalytic properties of mutant Pf-PLB.",

keywords = "Catalytic mechanism, Phospholipase B, Structure prediction",

author = "Yiwei Zhao and Fangyan Jiang and Chun Li and Dazhang Dai",

year = "2020",

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doi = "10.11949/0438-1157.20200527",

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T1 - 荧光假单胞杆菌磷脂酶B界面催化分子机制初探

AU - Zhao, Yiwei

AU - Jiang, Fangyan

AU - Li, Chun

AU - Dai, Dazhang

PY - 2020/9/1

Y1 - 2020/9/1

N2 - The physicochemical property and secondary structure of Pseudomonas fluorescens phospholipase B(Pf-PLB) were analyzed base on its primary structure, and the tertiary structure of Pf-PLB was predicated. A "lid-in, side-out" hypothesis model was put forward to illuminate the possible interfacial catalytic mechanism of Pf-PLB. The structure of Pf-PLB was modified by site-directed mutagenesis, and the proposed mechanism model was verified by combining with the catalytic properties of mutant Pf-PLB.

AB - The physicochemical property and secondary structure of Pseudomonas fluorescens phospholipase B(Pf-PLB) were analyzed base on its primary structure, and the tertiary structure of Pf-PLB was predicated. A "lid-in, side-out" hypothesis model was put forward to illuminate the possible interfacial catalytic mechanism of Pf-PLB. The structure of Pf-PLB was modified by site-directed mutagenesis, and the proposed mechanism model was verified by combining with the catalytic properties of mutant Pf-PLB.

KW - Catalytic mechanism

KW - Phospholipase B

KW - Structure prediction

UR - https://www.scopus.com/pages/publications/85091538939

U2 - 10.11949/0438-1157.20200527

DO - 10.11949/0438-1157.20200527

M3 - 文章

AN - SCOPUS:85091538939

SN - 0438-1157

VL - 71

SP - 4255

EP - 4259

JO - Huagong Xuebao/CIESC Journal

JF - Huagong Xuebao/CIESC Journal

IS - 9

ER -

荧光假单胞杆菌磷脂酶B界面催化分子机制初探

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