于 洋

主要研究方向：1）金属蛋白研究工具的开发；2）构建人工金属酶，实现新反应； 3）金属酶反应机理的研究。在国内外学术刊物上发表学术论文30余篇，主持3项自然科学基金等项目，参与多项重点研发项目，入选首批中国科协“青年人才托举工程”。

讲授“生物分子工程中的人工智能方法”、“Chemical Biology”、“制药工程专业实验”等本研课程，参编教材1本，主持校级教改项目2项，上线MOOC一门“Chemical Biology”，曾获校教学成果三等奖1项。指导学生获2次国际遗传工程机器竞赛（iGEM）金奖，全国大学生创新大赛北京赛区二等奖等省部级奖3次，获批国家级大学生创新创业训练计划优秀项目。2023年指导学生获北京市普通高校优秀本科生毕业设计（论文）。

从事蛋白质工程与生物催化研究，主要关注在自然界物质能量循环及生物合成应用中发挥重要作用的金属酶。围绕金属酶结构复杂、催化机制不清晰，以及天然酶性质无法满足应用需求的问题，开展以下研究
1.研究方向一 金属酶设计工具开发：发展基于人工智能的蛋白质设计、定向进化和评价方法，以及基于基因密码子扩展的非天然氨基酸插入工具。
2.研究方向二 复杂金属酶的设计：设计细胞色素c氧化酶、肼合酶等天然金属酶的模型蛋白，探索其催化机制。
3.研究方向三 生物催化工程：为拓展酶催化的应用，探索酶与金属配位化合物、功能材料偶联，构建化学-酶级联、酶-光偶联催化系统。

(27) Su Binbin, Yu Yang, Liu Haiping, and Li Chun. Engineering a Hetero-Bimetallic Azurin Photoenzyme for Photoredox Nitrite Reduction and SNO Adduct Formation, Chemistry – A European Journal, 2025, e202500143.                                    

(26) Wang Yanxia, Guo Bingchen, Chai Zhihua, Gao Ming, Li Yanlin, Yu Yang, and Huang Shengli. Enzyme immobilization in a Ru(N^N)3-modified covalent organic framework for photoenzymatic cascade catalysis, Journal of Materials Chemistry A,2024, 12(29): 18537-18543.                                    

(25) Sun Yiyang, Tang Yinian, Zhou Jing, Guo Bingchen, Yuan Feiyan, Yao Bo, Yu Yang, and Li Chun. Computational design of myoglobin-based carbene transferases for monoterpene derivatization, Biochemical and Biophysical Research Communications.,2024, 722150160.                                    

(24) Guo Bingchen, Wang Yanxia, Sheng Yukai, Zhao Xiaoxiao, Sun Yiyang, Zhou Jing, Wang Yingying, Zhou Xiaohong, Yu Yang, and Li Chun. Optimizing light-driven ene-reductase reactions with g-C3N4 and electron mediators, Applied Catalysis A: General,2024, 679119737.                                    

(23) Yuan Feiyan, Ding Jing, Sun Yiyang, Liang Jianhua, Luo Yunzi, and Yu Yang. Synthesis of Trifluoromethylated Monoterpenes by an Engineered Cytochrome P450, Chemistry – A European Journal,2024, 30(10): e202302936.                                    

(22) Wang Yan-Xia, Wang Ying, Li Jing, Yu Yang, Huang Sheng-Li, and Yang Guo-Yu. Ru(N^N)3-docked cationic covalent organic frameworks for enhanced sulfide and amine photooxidation, Dalton Trans.,2023, 52(39): 14100-14109.                                    

(21) Sheng Yukai, Guo Fang, Guo Bingchen, Wang Ning, Sun Yiyang, Liu Hu, Feng Xudong, Han Qing, Yu Yang, and Li Chun. Light-Driven CO2 Reduction with a Surface-Displayed Enzyme Cascade–C3N4 Hybrid, ACS Synthetic Biology,2023, 12(9): 2715-2724.                                    

(20)Yuan Feiyan, Su Binbin, Yu Yang, and Wang Jiangyun. Study and design of amino acid-based radical enzymes using unnatural amino acids, RSC Chemical Biology,2023, 4(6): 431-446.                                  

(19)Yu Yang, Marshall Nicholas M., Garner Dewain K., Nilges Mark J., and Lu Yi. Tuning reduction potentials of type 1 copper center in azurin by replacing a histidine ligand with its isostructural analogues, J. Inorg. Biochem.,2022, 234111863.                                    

(18) 马铮, 姜雨佳, 于洋, 李春. 含能化合物的生物合成反应. 生物加工过程 2022, 20, 465-475.                                    

(17) Jiang Yujia, Su Binbin, Chen Honghui, Zhang Tongtong, Liu Haiping, and Yu Yang. An engineered azurin with a lanthanide binding site capable of copper sensing, Biochemical and Biophysical Research Communications,2021, 56140-44.                                    

(16) 袁飞燕，于洋，李春. 基于非天然结构组件的人工酶设计与应用. 合成生物学 2020,1, 685-696.                                    

(15) 于洋, 王江云. 人工酶与定向进化的前沿与挑战, 科技导报. 2020, 38, 101-105.                                    

(14) Sun Wentao, Xue Haijie, Liu Hu, Lv Bo, Yu Yang, Wang Ying, Huang Meilan, and Li Chun. Controlling Chemo- and Regioselectivity of a Plant P450 in Yeast Cell toward Rare Licorice Triterpenoid Biosynthesis, ACS Catalysis,2020, 10(7): 4253-4260.                              

(13) Yu Yang, Liu Xiaohong, and Wang Jiangyun. Expansion of Redox Chemistry in Designer Metalloenzymes, Accounts of Chemical Research,2019, 52557-565.                                    

(12) 于洋.基于肌红蛋白的氧激活蛋白的理性设计, 生物加工过程 2019, 17, 23-28.                                    

(11) Yu Yang, Hu Cheng, Xia Lin, and Wang Jiangyun. Artificial Metalloenzyme Design with Unnatural Amino Acids and Non-Native Cofactors, ACS Catalysis,2018, 8(3): 1851-1863.                                    

(10) Chen Honghui, Su Binbin, Zhang Tongtong, Huang Aiping, Liu Haiping, Yu Yang, and Wang Jiangyun. Engineering the metal-binding loop at a type 1 copper center by circular permutation, RSC Advances,2017, 7(88): 56093-56098.                                    

(9) Hu Cheng, Yu Yang, and Wang Jiangyun. Improving artificial metalloenzymes' activity by optimizing electron transfer, Chemical Communications,2017, 53(30): 4173-4186.                                    

(8) Yu Yang, Cui Chang, Liu Xiaohong, Petrik Igor D., Wang Jiangyun, and Lu Yi. A Designed Metalloenzyme Achieving the Catalytic Rate of a Native Enzyme, Journal of the American Chemical Society,2015, 137(36): 11570-11573.

(7) Yu Yang, Zhou Qing, Wang Li, Liu Xiaohong, Zhang Wei, Hu Meirong, Dong Jianshu, Li Jiasong, Lv Xiaoxuan, Ouyang Hanlin, Li Han, Gao Feng, Gong Weimin, Lu Yi, and Wang Jiangyun. Significant improvement of oxidase activity through the genetic incorporation of a redox-active unnatural amino acid, Chemical Science,2015, 6(7): 3881-3885.

(6) Yu Yang, Lv Xiaoxuan, Li Jiasong, Zhou Qing, Cui Chang, Hosseinzadeh Parisa, Mukherjee Arnab, Nilges Mark J., Wang Jiangyun, and Lu Yi. Defining the Role of Tyrosine and Rational Tuning of Oxidase Activity by Genetic Incorporation of Unnatural Tyrosine Analogs, Journal of the American Chemical Society,2015, 137(14): 4594-4597.

(5) Lv Xiaoxuan, Yu Yang, Zhou Meng, Hu Cheng, Gao Feng, Li Jiasong, Liu Xiaohong, Deng Kai, Zheng Peng, Gong Weimin, Xia Andong, and Wang Jiangyun. Ultrafast Photoinduced Electron Transfer in Green Fluorescent Protein Bearing a Genetically Encoded Electron Acceptor, Journal of the American Chemical Society,2015, 137(23): 7270-7273.   

(4) Yu Yang, Mukherjee Arnab, Nilges Mark J., Hosseinzadeh Parisa, Miner Kyle D., and Lu Yi. Direct EPR Observation of a Tyrosyl Radical in a Functional Oxidase Model in Myoglobin during both H2O2 and O2 Reactions, Journal of the American Chemical Society,2014, 136(4): 1174-1177.                                    

(3) Liu Jing, Chakraborty Saumen, Hosseinzadeh Parisa, Yu Yang, Tian Shiliang, Petrik Igor, Bhagi Ambika, and Lu Yi. Metalloproteins Containing Cytochrome, Iron–Sulfur, or Copper Redox Centers, Chemical Reviews,2014, 114(8): 4366-4469.                                    

(2) Pan Yanchao, Jin Jing-Hua, Yu Yang, and Wang Jiangyun. Significant Enhancement of hPrx1 Chaperone Activity through Lysine Acetylation, ChemBioChem,2014, 15(12): 1773-1776.                            

(1) Liu Xiaohong, Yu Yang, Hu Cheng, Zhang Wei, Lu Yi, and Wang Jiangyun. Significant Increase of Oxidase Activity through the Genetic Incorporation of a Tyrosine-Histidine Cross-Link in a Myoglobin Model of Heme-Copper Oxidase, Angewandte Chemie International Edition,2012, 51(18): 4312-4316.