Ultrasensitive flow injection chemiluminescence detection of DNA hybridization using nanoCuS tags

Caifeng Ding; Hua Zhong; Shusheng Zhang

doi:10.1016/j.bios.2007.12.005

Ultrasensitive flow injection chemiluminescence detection of DNA hybridization using nanoCuS tags

Caifeng Ding, Hua Zhong, Shusheng Zhang^*

^*Corresponding author for this work

Ministry of Education in China

Research output: Contribution to journal › Article › peer-review

59 Citations (Scopus)

Abstract

A novel and sensitive biosensor for the determination of short sequence of DNA based on flow injection (FI)-chemiluminescence (CL) system of luminol-H₂O₂-Cu²⁺ was developed in the present work. The DNA probe labeled with copper sulfide nanoparticles (CuS NPs) could hybridize with target DNA immobilized on glass-carbon electrode (GCE). The hybridization events were monitored by the CL intensity of luminol-H₂O₂-Cu²⁺ after the cupric ions was dissolved from the hybrids. A preconcentration process of cupric ions was performed by anodic stripping voltammetry (ASV) technology to improve the sensitivity of the biosensor. Under the optimum conditions, the CL intensity was proportional to the concentration of target DNA in the range of 2.0 × 10^-12-1.0 × 10^-10 M. A detection limit of 5.5 × 10^-13 M of target DNA was achieved. The CL intensity of two-base mismatched sequences and noncomplementary sequences were also detected. The experiments indicated that two-base mismatched sequences showed weaker CL intensity and noncomplementary sequences gave no response at all.

Original language	English
Pages (from-to)	1314-1318
Number of pages	5
Journal	Biosensors and Bioelectronics
Volume	23
Issue number	8
DOIs	https://doi.org/10.1016/j.bios.2007.12.005
Publication status	Published - 14 Mar 2008
Externally published	Yes

Keywords

CuS nanoparticles
DNA biosensor
Flow injection chemiluminescence
Preconcentration

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10.1016/j.bios.2007.12.005

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title = "Ultrasensitive flow injection chemiluminescence detection of DNA hybridization using nanoCuS tags",

abstract = "A novel and sensitive biosensor for the determination of short sequence of DNA based on flow injection (FI)-chemiluminescence (CL) system of luminol-H2O2-Cu2+ was developed in the present work. The DNA probe labeled with copper sulfide nanoparticles (CuS NPs) could hybridize with target DNA immobilized on glass-carbon electrode (GCE). The hybridization events were monitored by the CL intensity of luminol-H2O2-Cu2+ after the cupric ions was dissolved from the hybrids. A preconcentration process of cupric ions was performed by anodic stripping voltammetry (ASV) technology to improve the sensitivity of the biosensor. Under the optimum conditions, the CL intensity was proportional to the concentration of target DNA in the range of 2.0 × 10-12-1.0 × 10-10 M. A detection limit of 5.5 × 10-13 M of target DNA was achieved. The CL intensity of two-base mismatched sequences and noncomplementary sequences were also detected. The experiments indicated that two-base mismatched sequences showed weaker CL intensity and noncomplementary sequences gave no response at all.",

keywords = "CuS nanoparticles, DNA biosensor, Flow injection chemiluminescence, Preconcentration",

author = "Caifeng Ding and Hua Zhong and Shusheng Zhang",

year = "2008",

month = mar,

day = "14",

doi = "10.1016/j.bios.2007.12.005",

language = "English",

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journal = "Biosensors and Bioelectronics",

issn = "0956-5663",

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T1 - Ultrasensitive flow injection chemiluminescence detection of DNA hybridization using nanoCuS tags

AU - Ding, Caifeng

AU - Zhong, Hua

AU - Zhang, Shusheng

PY - 2008/3/14

Y1 - 2008/3/14

N2 - A novel and sensitive biosensor for the determination of short sequence of DNA based on flow injection (FI)-chemiluminescence (CL) system of luminol-H2O2-Cu2+ was developed in the present work. The DNA probe labeled with copper sulfide nanoparticles (CuS NPs) could hybridize with target DNA immobilized on glass-carbon electrode (GCE). The hybridization events were monitored by the CL intensity of luminol-H2O2-Cu2+ after the cupric ions was dissolved from the hybrids. A preconcentration process of cupric ions was performed by anodic stripping voltammetry (ASV) technology to improve the sensitivity of the biosensor. Under the optimum conditions, the CL intensity was proportional to the concentration of target DNA in the range of 2.0 × 10-12-1.0 × 10-10 M. A detection limit of 5.5 × 10-13 M of target DNA was achieved. The CL intensity of two-base mismatched sequences and noncomplementary sequences were also detected. The experiments indicated that two-base mismatched sequences showed weaker CL intensity and noncomplementary sequences gave no response at all.

AB - A novel and sensitive biosensor for the determination of short sequence of DNA based on flow injection (FI)-chemiluminescence (CL) system of luminol-H2O2-Cu2+ was developed in the present work. The DNA probe labeled with copper sulfide nanoparticles (CuS NPs) could hybridize with target DNA immobilized on glass-carbon electrode (GCE). The hybridization events were monitored by the CL intensity of luminol-H2O2-Cu2+ after the cupric ions was dissolved from the hybrids. A preconcentration process of cupric ions was performed by anodic stripping voltammetry (ASV) technology to improve the sensitivity of the biosensor. Under the optimum conditions, the CL intensity was proportional to the concentration of target DNA in the range of 2.0 × 10-12-1.0 × 10-10 M. A detection limit of 5.5 × 10-13 M of target DNA was achieved. The CL intensity of two-base mismatched sequences and noncomplementary sequences were also detected. The experiments indicated that two-base mismatched sequences showed weaker CL intensity and noncomplementary sequences gave no response at all.

KW - CuS nanoparticles

KW - DNA biosensor

KW - Flow injection chemiluminescence

KW - Preconcentration

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EP - 1318

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 8

ER -

Ultrasensitive flow injection chemiluminescence detection of DNA hybridization using nanoCuS tags

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Keywords

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