Two-triplet CGA repeats impede DNA replication in bacteriophage M13 in Escherichia coli

Xuefeng Pan

doi:10.1016/j.micres.2003.12.001

Two-triplet CGA repeats impede DNA replication in bacteriophage M13 in Escherichia coli

Xuefeng Pan^*

^*Corresponding author for this work

CAS - Institute of Microbiology

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Expansion and contraction instabilities associated with CAG, CGG, GAA and CGA (GAC) repeats propagation cause more than a dozen human genetic diseases and cancers. In this work, the propagation behavior of a bacteriophage M13 carrying a calf prochymosin cDNA fragment with a (CGA)₂ repeat in a small hairpin forming region is reported. Such a M13 derivative when propagated in Escherichia coli, produces small plaques by decreasing phage yield and also mitigates the inhibition on host cell growth, compared to those control bacteriophages either containing a "CTGCTA" sequence or wildtype, suggesting that CGA₂ repeat impedes DNA replication in vivo. Moreover, an increased internal free energy is found associated with (CGA) ₂ sequence compared to those "CTGCTA" and wildtype, which ruled out a possibility of CGA₂ repeat effects on propagation is through influencing the hairpin structure formation.

Original language	English
Pages (from-to)	97-102
Number of pages	6
Journal	Microbiological Research
Volume	159
Issue number	2
DOIs	https://doi.org/10.1016/j.micres.2003.12.001
Publication status	Published - 30 Jun 2004
Externally published	Yes

Keywords

CGA repeats - bacteriophage M13
Site-directed mutagenesis - DNA replication

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10.1016/j.micres.2003.12.001

Cite this

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title = "Two-triplet CGA repeats impede DNA replication in bacteriophage M13 in Escherichia coli",

abstract = "Expansion and contraction instabilities associated with CAG, CGG, GAA and CGA (GAC) repeats propagation cause more than a dozen human genetic diseases and cancers. In this work, the propagation behavior of a bacteriophage M13 carrying a calf prochymosin cDNA fragment with a (CGA)2 repeat in a small hairpin forming region is reported. Such a M13 derivative when propagated in Escherichia coli, produces small plaques by decreasing phage yield and also mitigates the inhibition on host cell growth, compared to those control bacteriophages either containing a {"}CTGCTA{"} sequence or wildtype, suggesting that CGA2 repeat impedes DNA replication in vivo. Moreover, an increased internal free energy is found associated with (CGA) 2 sequence compared to those {"}CTGCTA{"} and wildtype, which ruled out a possibility of CGA2 repeat effects on propagation is through influencing the hairpin structure formation.",

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author = "Xuefeng Pan",

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T1 - Two-triplet CGA repeats impede DNA replication in bacteriophage M13 in Escherichia coli

AU - Pan, Xuefeng

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N2 - Expansion and contraction instabilities associated with CAG, CGG, GAA and CGA (GAC) repeats propagation cause more than a dozen human genetic diseases and cancers. In this work, the propagation behavior of a bacteriophage M13 carrying a calf prochymosin cDNA fragment with a (CGA)2 repeat in a small hairpin forming region is reported. Such a M13 derivative when propagated in Escherichia coli, produces small plaques by decreasing phage yield and also mitigates the inhibition on host cell growth, compared to those control bacteriophages either containing a "CTGCTA" sequence or wildtype, suggesting that CGA2 repeat impedes DNA replication in vivo. Moreover, an increased internal free energy is found associated with (CGA) 2 sequence compared to those "CTGCTA" and wildtype, which ruled out a possibility of CGA2 repeat effects on propagation is through influencing the hairpin structure formation.

AB - Expansion and contraction instabilities associated with CAG, CGG, GAA and CGA (GAC) repeats propagation cause more than a dozen human genetic diseases and cancers. In this work, the propagation behavior of a bacteriophage M13 carrying a calf prochymosin cDNA fragment with a (CGA)2 repeat in a small hairpin forming region is reported. Such a M13 derivative when propagated in Escherichia coli, produces small plaques by decreasing phage yield and also mitigates the inhibition on host cell growth, compared to those control bacteriophages either containing a "CTGCTA" sequence or wildtype, suggesting that CGA2 repeat impedes DNA replication in vivo. Moreover, an increased internal free energy is found associated with (CGA) 2 sequence compared to those "CTGCTA" and wildtype, which ruled out a possibility of CGA2 repeat effects on propagation is through influencing the hairpin structure formation.

KW - CGA repeats - bacteriophage M13

KW - Site-directed mutagenesis - DNA replication

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DO - 10.1016/j.micres.2003.12.001

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JO - Microbiological Research

JF - Microbiological Research

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ER -

Two-triplet CGA repeats impede DNA replication in bacteriophage M13 in Escherichia coli

Abstract

Keywords

UN SDGs

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