The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors

Hui Wang; Boyuan Li; Linyu Zuo; Bo Wang; Yan Yan; Kai Tian; Rong Zhou; Chenlu Wang; Xizi Chen; Yongpeng Jiang; Haonan Zheng; Fangfei Qin; Bin Zhang; Yang Yu; Chao Pei Liu; Yanhui Xu; Juntao Gao; Zhi Qi; Wulan Deng; Xiong Ji

doi:10.1038/s41467-022-33433-3

The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors

Hui Wang, Boyuan Li, Linyu Zuo, Bo Wang, Yan Yan, Kai Tian, Rong Zhou, Chenlu Wang, Xizi Chen, Yongpeng Jiang, Haonan Zheng, Fangfei Qin, Bin Zhang, Yang Yu, Chao Pei Liu, Yanhui Xu, Juntao Gao, Zhi Qi, Wulan Deng, Xiong Ji^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

RNA polymerase II (Pol II) apparatuses are compartmentalized into transcriptional clusters. Whether protein factors control these clusters remains unknown. In this study, we find that the ATPase-associated with diverse cellular activities (AAA +) ATPase RUVBL2 co-occupies promoters with Pol II and various transcription factors. RUVBL2 interacts with unphosphorylated Pol II in chromatin to promote RPB1 carboxy-terminal domain (CTD) clustering and transcription initiation. Rapid depletion of RUVBL2 leads to a decrease in the number of Pol II clusters and inhibits nascent RNA synthesis, and tethering RUVBL2 to an active promoter enhances Pol II clustering at the promoter. We also identify target genes that are directly linked to the RUVBL2-Pol II axis. Many of these genes are hallmarks of cancers and encode proteins with diverse cellular functions. Our results demonstrate an emerging activity for RUVBL2 in regulating Pol II cluster formation in the nucleus.

Original language	English
Article number	5703
Journal	Nature Communications
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/s41467-022-33433-3
Publication status	Published - Dec 2022
Externally published	Yes

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Wang, H., Li, B., Zuo, L., Wang, B., Yan, Y., Tian, K., Zhou, R., Wang, C., Chen, X., Jiang, Y., Zheng, H., Qin, F., Zhang, B., Yu, Y., Liu, C. P., Xu, Y., Gao, J., Qi, Z., Deng, W., & Ji, X. (2022). The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors. Nature Communications, 13(1), Article 5703. https://doi.org/10.1038/s41467-022-33433-3

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title = "The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors",

abstract = "RNA polymerase II (Pol II) apparatuses are compartmentalized into transcriptional clusters. Whether protein factors control these clusters remains unknown. In this study, we find that the ATPase-associated with diverse cellular activities (AAA +) ATPase RUVBL2 co-occupies promoters with Pol II and various transcription factors. RUVBL2 interacts with unphosphorylated Pol II in chromatin to promote RPB1 carboxy-terminal domain (CTD) clustering and transcription initiation. Rapid depletion of RUVBL2 leads to a decrease in the number of Pol II clusters and inhibits nascent RNA synthesis, and tethering RUVBL2 to an active promoter enhances Pol II clustering at the promoter. We also identify target genes that are directly linked to the RUVBL2-Pol II axis. Many of these genes are hallmarks of cancers and encode proteins with diverse cellular functions. Our results demonstrate an emerging activity for RUVBL2 in regulating Pol II cluster formation in the nucleus.",

author = "Hui Wang and Boyuan Li and Linyu Zuo and Bo Wang and Yan Yan and Kai Tian and Rong Zhou and Chenlu Wang and Xizi Chen and Yongpeng Jiang and Haonan Zheng and Fangfei Qin and Bin Zhang and Yang Yu and Liu, {Chao Pei} and Yanhui Xu and Juntao Gao and Zhi Qi and Wulan Deng and Xiong Ji",

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doi = "10.1038/s41467-022-33433-3",

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AU - Wang, Hui

AU - Li, Boyuan

AU - Zuo, Linyu

AU - Wang, Bo

AU - Yan, Yan

AU - Tian, Kai

AU - Zhou, Rong

AU - Wang, Chenlu

AU - Chen, Xizi

AU - Jiang, Yongpeng

AU - Zheng, Haonan

AU - Qin, Fangfei

AU - Zhang, Bin

AU - Yu, Yang

AU - Liu, Chao Pei

AU - Xu, Yanhui

AU - Gao, Juntao

AU - Qi, Zhi

AU - Deng, Wulan

AU - Ji, Xiong

PY - 2022/12

Y1 - 2022/12

N2 - RNA polymerase II (Pol II) apparatuses are compartmentalized into transcriptional clusters. Whether protein factors control these clusters remains unknown. In this study, we find that the ATPase-associated with diverse cellular activities (AAA +) ATPase RUVBL2 co-occupies promoters with Pol II and various transcription factors. RUVBL2 interacts with unphosphorylated Pol II in chromatin to promote RPB1 carboxy-terminal domain (CTD) clustering and transcription initiation. Rapid depletion of RUVBL2 leads to a decrease in the number of Pol II clusters and inhibits nascent RNA synthesis, and tethering RUVBL2 to an active promoter enhances Pol II clustering at the promoter. We also identify target genes that are directly linked to the RUVBL2-Pol II axis. Many of these genes are hallmarks of cancers and encode proteins with diverse cellular functions. Our results demonstrate an emerging activity for RUVBL2 in regulating Pol II cluster formation in the nucleus.

AB - RNA polymerase II (Pol II) apparatuses are compartmentalized into transcriptional clusters. Whether protein factors control these clusters remains unknown. In this study, we find that the ATPase-associated with diverse cellular activities (AAA +) ATPase RUVBL2 co-occupies promoters with Pol II and various transcription factors. RUVBL2 interacts with unphosphorylated Pol II in chromatin to promote RPB1 carboxy-terminal domain (CTD) clustering and transcription initiation. Rapid depletion of RUVBL2 leads to a decrease in the number of Pol II clusters and inhibits nascent RNA synthesis, and tethering RUVBL2 to an active promoter enhances Pol II clustering at the promoter. We also identify target genes that are directly linked to the RUVBL2-Pol II axis. Many of these genes are hallmarks of cancers and encode proteins with diverse cellular functions. Our results demonstrate an emerging activity for RUVBL2 in regulating Pol II cluster formation in the nucleus.

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VL - 13

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The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors

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