TY - JOUR
T1 - The effects of c60(c(cooh)2)2-fitc on proliferation and differentiation of human mesenchymal stem cells in vitro
AU - Li, Juan
AU - Song, Yan
AU - Liu, Xiaoxiao
AU - Zhang, Mingyi
AU - He, Rui
AU - Chang, Yanan
AU - Jin, Junjiang
AU - Xing, Geng Mei
AU - Zhang, Jun
PY - 2013/10
Y1 - 2013/10
N2 - As manufactured nanoparticles, fullerene nanoparticles were used as the model to research the manufactured nanoparticles entering into cells and hence have been rapidly developed for biomedical uses. Human mesenchymal stem cells (hMSCs) have become the most widely used seeding cells in tissue engineering because they are readily obtained without ethical problems and are multipotent with regard to adipogenic, chondrogenic, and osteogenic lineages. Because of their favorable biological and cellular activities, C60 carboxyl derivatives are among the most widely studied C60 derivatives. FITC labeled C60 (C(COOH)2)2 nanoparticles were charactered by FTIR, ESI-MS, XPS and DLS. The effects of C60 (C(COOH)2)2-FITC on proliferation and differentiation of human mesenchymal stem cells (hMSCs) in vitro were observed. The fullerene nanoparticles are quickly internalized by the cells and they had low toxicity to proliferation of hMSCs. The C60(C(COOH)2)2 nanoparticles could promote cell proliferation, enhance osteoclast differentiation of hMSCs.
AB - As manufactured nanoparticles, fullerene nanoparticles were used as the model to research the manufactured nanoparticles entering into cells and hence have been rapidly developed for biomedical uses. Human mesenchymal stem cells (hMSCs) have become the most widely used seeding cells in tissue engineering because they are readily obtained without ethical problems and are multipotent with regard to adipogenic, chondrogenic, and osteogenic lineages. Because of their favorable biological and cellular activities, C60 carboxyl derivatives are among the most widely studied C60 derivatives. FITC labeled C60 (C(COOH)2)2 nanoparticles were charactered by FTIR, ESI-MS, XPS and DLS. The effects of C60 (C(COOH)2)2-FITC on proliferation and differentiation of human mesenchymal stem cells (hMSCs) in vitro were observed. The fullerene nanoparticles are quickly internalized by the cells and they had low toxicity to proliferation of hMSCs. The C60(C(COOH)2)2 nanoparticles could promote cell proliferation, enhance osteoclast differentiation of hMSCs.
KW - Fluorescence Labeled Fullerene
KW - Fullerene Carboxyl Derivatives
KW - Human Mesenchymal Stem Cells.
UR - http://www.scopus.com/inward/record.url?scp=84889065542&partnerID=8YFLogxK
U2 - 10.1166/jnn.2013.7166
DO - 10.1166/jnn.2013.7166
M3 - Article
C2 - 24245108
AN - SCOPUS:84889065542
SN - 1533-4880
VL - 13
SP - 6517
EP - 6521
JO - Journal of Nanoscience and Nanotechnology
JF - Journal of Nanoscience and Nanotechnology
IS - 10
ER -