The Construction and Mechanism of SPCE/Cu₂O@MWCNTs Electrochemical Sensor for Menthone Detection for Epileptic Seizures Prediction

Prediction of seizures is critical for the effective management and treatment of epileptic disorders. The epileptic patients generates certain biomarker before the occurance of seizure, which are considered as the potential biomarkers for prediction. However, research on the detection of these biomarkers remains limited, and the detection process is still challenging. Herein, an electrochemical sensor for detecting menthone, a biomarker associated with epilepstic seizure is reported. The menthone electrochemical sensor is based on the screen-printed carbon electrode (SPCE) modified with a Cu₂O@MWCNTs nanomaterial. The electrode exhibits the detection limit of 0.3 mM and the sensitivity of 88.243 µA·mM⁻¹·cm⁻². Additionally, the SPCE/Cu₂O@MWCNTs electrode demonstrates a good linearship with menthone concentration in phosphate buffered saline​​ (PBS). Cell assay results further confirm the excellent biocompatibility of the SPCE/Cu₂O@MWCNTs electrode, highlighting its potential for its utilization in the real biosamples. Through the mechanism study, the reaction mechanism between menthone and the sensing material is proposed. This electrode not only provides a reliable and cost-effective method for predicting epileptic seizures, thus paving the way for advancements in electrochemical biosensing technologies.