Temperature-sensitive protein expression in protocells

Haiyang Jia; Michael Heymann; Tobias Härtel; Lei Kai; Petra Schwille

doi:10.1039/c9cc02734c

Temperature-sensitive protein expression in protocells

Haiyang Jia, Michael Heymann, Tobias Härtel, Lei Kai, Petra Schwille^*

^*Corresponding author for this work

Max Planck Institute of Biochemistry

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

We engineered a synthetic temperature regulation toolbox to enable protocells to sense and respond to heat, utilizing RNA thermometers. The thermo-sensitive protocells were generated by encapsulating temperature feedback transcription/translation machinery in droplets. Based on these temperature-sensing devices, the protocells can be operated with logic AND gates, differentially processing temperature stimuli into biological signals.

Original language	English
Pages (from-to)	6421-6424
Number of pages	4
Journal	Chemical Communications
Volume	55
Issue number	45
DOIs	https://doi.org/10.1039/c9cc02734c
Publication status	Published - 2019
Externally published	Yes

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10.1039/c9cc02734c

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title = "Temperature-sensitive protein expression in protocells",

abstract = "We engineered a synthetic temperature regulation toolbox to enable protocells to sense and respond to heat, utilizing RNA thermometers. The thermo-sensitive protocells were generated by encapsulating temperature feedback transcription/translation machinery in droplets. Based on these temperature-sensing devices, the protocells can be operated with logic AND gates, differentially processing temperature stimuli into biological signals.",

author = "Haiyang Jia and Michael Heymann and Tobias H{\"a}rtel and Lei Kai and Petra Schwille",

note = "Publisher Copyright: {\textcopyright} 2019 The Royal Society of Chemistry.",

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AU - Jia, Haiyang

AU - Heymann, Michael

AU - Härtel, Tobias

AU - Kai, Lei

AU - Schwille, Petra

PY - 2019

Y1 - 2019

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AB - We engineered a synthetic temperature regulation toolbox to enable protocells to sense and respond to heat, utilizing RNA thermometers. The thermo-sensitive protocells were generated by encapsulating temperature feedback transcription/translation machinery in droplets. Based on these temperature-sensing devices, the protocells can be operated with logic AND gates, differentially processing temperature stimuli into biological signals.

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U2 - 10.1039/c9cc02734c

DO - 10.1039/c9cc02734c

M3 - Article

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SN - 1359-7345

VL - 55

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JO - Chemical Communications

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Temperature-sensitive protein expression in protocells

Abstract

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