Study on the interaction of metallothionein with glutathione using ferrocenyl-labeled glutathione as an electrochemical probe

Ping Chen, Yong Peng, Yuan Qiang Hao, You Nian Liu*, Juan Li

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The interaction between glutathione (GSH) and metallothionein (MT) plays a critical role in metal release from MT in life processes. In this paper, the interaction was measured directly by voltammetric method at the MT-modified electrode, due to the attachment of the redox-active ferrocene moiety to GSH. Particularly, the covalent attachment of ferrocene moiety to GSH does not significantly perturb the GSH structure and its binding behavior with MT. The GSH/MT stoichiometry and binding constant Ka were obtained by cyclic voltammetric method and the values are 1.78 ± 0.15 and 5.44 ± 0.43 ×106 M-1, respectively. In addition, zinc release from MT in the presence of glutathione or glutathione disulfide (GSSG) was also studied by means of differential pulse voltammetry. The difference in binding constant between Zn-MT and GSH-MT complexes explains the inhibition of GSH on the zinc release from MT.

Original languageEnglish
Pages (from-to)8227-8235
Number of pages9
JournalInternational Journal of Electrochemical Science
Volume8
Issue number6
Publication statusPublished - Jun 2013
Externally publishedYes

Keywords

  • Ferrocenyl-labeled glutathione
  • Interaction behavior
  • Metallothionein
  • Zinc ion release

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