TY - JOUR
T1 - Simulated microgravity affects growth of Escherichia coli and recombinant β-D-glucuronidase production
AU - Xiang, Liang
AU - Qi, Feng
AU - Dai, Dazhang
AU - Li, Chun
AU - Jiang, Yuanda
PY - 2010/10
Y1 - 2010/10
N2 - Effects of simulated microgravity (SMG) on bacteria have been studied in various aspects. However, few reports are available about production of recombinant protein expressed by bacteria in SMG. In this study growth of E. coli BL21 (DE3) cells transformed with pET-28a (+)-pgus in double-axis clinostat that could model low shear SMG environment and the recombinant β-D-glucuronidase (PGUS) expression have been investigated. Results showed that the cell dry weights in SMG were 16.47%, 38.06%, and 28.79% more than normal gravity (NG) control, and the efficiency of the recombinant PGUS expression in SMG were 18.33%, 19.36%, and 33.42% higher than that in NG at 19 °C, 28 °C, and 37 °C, respectively (P<0.05).
AB - Effects of simulated microgravity (SMG) on bacteria have been studied in various aspects. However, few reports are available about production of recombinant protein expressed by bacteria in SMG. In this study growth of E. coli BL21 (DE3) cells transformed with pET-28a (+)-pgus in double-axis clinostat that could model low shear SMG environment and the recombinant β-D-glucuronidase (PGUS) expression have been investigated. Results showed that the cell dry weights in SMG were 16.47%, 38.06%, and 28.79% more than normal gravity (NG) control, and the efficiency of the recombinant PGUS expression in SMG were 18.33%, 19.36%, and 33.42% higher than that in NG at 19 °C, 28 °C, and 37 °C, respectively (P<0.05).
KW - E. coli
KW - Expression
KW - Normal gravity (NG)
KW - Recombinant PGUS
KW - Simulated microgravity (SMG)
UR - https://www.scopus.com/pages/publications/77954887220
U2 - 10.1007/s12010-009-8836-0
DO - 10.1007/s12010-009-8836-0
M3 - Article
C2 - 19921492
AN - SCOPUS:77954887220
SN - 0273-2289
VL - 162
SP - 654
EP - 661
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
IS - 3
ER -