Self-assembly of toroidal magnetic microstructures towards in vitro cell structures

Masaru Takeuchi, Mamoru Hattori, Akihiko Ichikawa, Kenichi Ohara, Masahiro Nakajima, Toshio Fukuda, Yasuhisa Hasegawa, Qiang Huang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

7 Citations (Scopus)

Abstract

In this paper, we propose a new method to assemble microstructures with biological cells towards in vitro 3D cellular structures. The proposed assembly method uses self-assembly process of magnetized toroidal microstructures. Biocompatible toroidal hydrogel microstructures are prepared by electrodeposition method, and ferrite particles are put on the fabricated structures using poly-L-lysine (PLL). The microstructures are magnetized by the magnetic field at 3 T, and assembled by the magnetic self-assembly process. Biological cells were encapsulated in the microstructures and cultured to achieve high density of cells inside structures. The magnetized microstructures were assembled automatically. The magnetic force generated from the ferrite embedded microstructures was estimated and compared to the fluid resistance applied to the microstructures. The proposed method can be applied to achieve 3D in vitro cell structures with vascular networks for tissue engineering applications.

Original languageEnglish
Title of host publicationIROS 2016 - 2016 IEEE/RSJ International Conference on Intelligent Robots and Systems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages2578-2583
Number of pages6
ISBN (Electronic)9781509037629
DOIs
Publication statusPublished - 28 Nov 2016
Event2016 IEEE/RSJ International Conference on Intelligent Robots and Systems, IROS 2016 - Daejeon, Korea, Republic of
Duration: 9 Oct 201614 Oct 2016

Publication series

NameIEEE International Conference on Intelligent Robots and Systems
Volume2016-November
ISSN (Print)2153-0858
ISSN (Electronic)2153-0866

Conference

Conference2016 IEEE/RSJ International Conference on Intelligent Robots and Systems, IROS 2016
Country/TerritoryKorea, Republic of
CityDaejeon
Period9/10/1614/10/16

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