Selection and identification of H1N1 influenza virus hemagglutinin inhibitory peptides

Chong Wen Wang; Xiao Ran Ding; Jing Yang; Xin Xiu Deng; Chan Gao; Zhi Quan Feng; Dan Dan Lu; Sheng Qi Wang

Selection and identification of H1N1 influenza virus hemagglutinin inhibitory peptides

Chong Wen Wang, Xiao Ran Ding, Jing Yang, Xin Xiu Deng, Chan Gao, Zhi Quan Feng, Dan Dan Lu, Sheng Qi Wang

Research output: Contribution to journal › Article › peer-review

Abstract

Objective Using the influenza virus hemagglutinin (HA) as the target to screen for novel anti-influenza polypeptide drugs. Methods The HA binding peptides were screened out through affinity selection from a 12-peptide phage library, and the anti-H1N1 activity was evaluated at MDCK cell and chicken embryo(ovo). Results Nine HA binding peptides were finally obtained, and the H6 peptide was found having significant antiviral activity against H1N1. Its IC₅₀ against two strains of H1N1, A/FM1/1/47 (H1N1) and A/PPR8/34(H 1 N 1), were 37. 3 and 48. 5 μmoZL respectively determined by cytopathic effect (CPE)test, and 26. 7 and 33. 4 μmoI/L respectively measured by ovo antiviral experiment. Conclusion These results showed that H6 might be a potential herapeu icdrug for H1N1 infecion.

Original language	English
Pages (from-to)	48-52
Number of pages	5
Journal	Journal of International Pharmaceutical Research
Volume	40
Issue number	1
Publication status	Published - 2013
Externally published	Yes

Keywords

HA binding peptide
Influenza virus
Inhibitor
Phage display

Cite this

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title = "Selection and identification of H1N1 influenza virus hemagglutinin inhibitory peptides",

abstract = "Objective Using the influenza virus hemagglutinin (HA) as the target to screen for novel anti-influenza polypeptide drugs. Methods The HA binding peptides were screened out through affinity selection from a 12-peptide phage library, and the anti-H1N1 activity was evaluated at MDCK cell and chicken embryo(ovo). Results Nine HA binding peptides were finally obtained, and the H6 peptide was found having significant antiviral activity against H1N1. Its IC50 against two strains of H1N1, A/FM1/1/47 (H1N1) and A/PPR8/34(H 1 N 1), were 37. 3 and 48. 5 μmoZL respectively determined by cytopathic effect (CPE)test, and 26. 7 and 33. 4 μmoI/L respectively measured by ovo antiviral experiment. Conclusion These results showed that H6 might be a potential herapeu icdrug for H1N1 infecion.",

keywords = "HA binding peptide, Influenza virus, Inhibitor, Phage display",

author = "Wang, {Chong Wen} and Ding, {Xiao Ran} and Jing Yang and Deng, {Xin Xiu} and Chan Gao and Feng, {Zhi Quan} and Lu, {Dan Dan} and Wang, {Sheng Qi}",

year = "2013",

language = "English",

volume = "40",

pages = "48--52",

journal = "Journal of International Pharmaceutical Research",

issn = "1674-0440",

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number = "1",

}

TY - JOUR

T1 - Selection and identification of H1N1 influenza virus hemagglutinin inhibitory peptides

AU - Wang, Chong Wen

AU - Ding, Xiao Ran

AU - Yang, Jing

AU - Deng, Xin Xiu

AU - Gao, Chan

AU - Feng, Zhi Quan

AU - Lu, Dan Dan

AU - Wang, Sheng Qi

PY - 2013

Y1 - 2013

N2 - Objective Using the influenza virus hemagglutinin (HA) as the target to screen for novel anti-influenza polypeptide drugs. Methods The HA binding peptides were screened out through affinity selection from a 12-peptide phage library, and the anti-H1N1 activity was evaluated at MDCK cell and chicken embryo(ovo). Results Nine HA binding peptides were finally obtained, and the H6 peptide was found having significant antiviral activity against H1N1. Its IC50 against two strains of H1N1, A/FM1/1/47 (H1N1) and A/PPR8/34(H 1 N 1), were 37. 3 and 48. 5 μmoZL respectively determined by cytopathic effect (CPE)test, and 26. 7 and 33. 4 μmoI/L respectively measured by ovo antiviral experiment. Conclusion These results showed that H6 might be a potential herapeu icdrug for H1N1 infecion.

AB - Objective Using the influenza virus hemagglutinin (HA) as the target to screen for novel anti-influenza polypeptide drugs. Methods The HA binding peptides were screened out through affinity selection from a 12-peptide phage library, and the anti-H1N1 activity was evaluated at MDCK cell and chicken embryo(ovo). Results Nine HA binding peptides were finally obtained, and the H6 peptide was found having significant antiviral activity against H1N1. Its IC50 against two strains of H1N1, A/FM1/1/47 (H1N1) and A/PPR8/34(H 1 N 1), were 37. 3 and 48. 5 μmoZL respectively determined by cytopathic effect (CPE)test, and 26. 7 and 33. 4 μmoI/L respectively measured by ovo antiviral experiment. Conclusion These results showed that H6 might be a potential herapeu icdrug for H1N1 infecion.

KW - HA binding peptide

KW - Influenza virus

KW - Inhibitor

KW - Phage display

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M3 - Article

AN - SCOPUS:84946169535

SN - 1674-0440

VL - 40

SP - 48

EP - 52

JO - Journal of International Pharmaceutical Research

JF - Journal of International Pharmaceutical Research

IS - 1

ER -

Selection and identification of H1N1 influenza virus hemagglutinin inhibitory peptides

Abstract

Keywords

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