@inproceedings{ebf9632e8e7f47c588855825d895f754,
title = "Screening and sequencing monoclonal antibody at single-cell level",
abstract = "Acquisition of the genes encoding variable regions of paired heavy and light chains (VH:VL) is crucial, but it is a labor and cost-intensive process in traditional methods. This study presents a novel method in which all processing steps for acquiring natively paired VH:VL genes from single cells are finished in a single microfluidic chip. The microfluidic chip performs single-cell trap/in situ fluorescent examination of antibody specificity/cell lysis/gene amplification all at single-cell level. By a proof-of-concept validation of efficiently acquiring paired VH:VL genes of anti-RBD (which is a key protein of SARS-CoV-2 virus) mAbs from single hybridomas, the microfluidic chip has been proved capable of remarkably improving cell loss/human labor/time cost, and more importantly, determinacy of native VH:VL genes pairing which is one of the most decisive factors of effectiveness for antibody discovery.",
keywords = "Heavy and light chains, Microfluidic chip, Monoclonal antibody, Single-cell",
author = "Weikai Zhang and Zewen Wei and Qin Li",
note = "Publisher Copyright: {\textcopyright} 2021 SPIE.; Optics in Health Care and Biomedical Optics XI 2021 ; Conference date: 10-10-2021 Through 12-10-2021",
year = "2021",
doi = "10.1117/12.2601468",
language = "English",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "SPIE",
editor = "Qingming Luo and Xingde Li and Ying Gu and Dan Zhu",
booktitle = "Optics in Health Care and Biomedical Optics XI",
address = "United States",
}