Rufibacter glacialis sp. Nov., a psychrotolerant bacterium isolated from glacier soil

Qing Liu; Hong Can Liu; Jian Li Zhang; Yu Guang Zhou; Yu Hua Xin

doi:10.1099/ijsem.0.000717

Rufibacter glacialis sp. Nov., a psychrotolerant bacterium isolated from glacier soil

Qing Liu
, Hong Can Liu
, Jian Li Zhang^*
, Yu Guang Zhou
, Yu Hua Xin

^*Corresponding author for this work

School of Life Science

CAS - Institute of Microbiology

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

A Gram-stain-negative, rod-shaped, red-pigmented bacterium (MDT1-10-3^T) was isolated from Midui glacier in Tibet, China. Cells were aerobic and psychrotolerant (growth occurred at 4-25 8C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that it was a member of the genus Rufibacter, with Rufibacter immobilis MCC P1^T(96.7% similarity) as its closest phylogenetic relative. MK-7 was the predominant respiratory menaquinone. The major cellular fatty acids were summed feature 4 (iso-C_{17 : 1}I and/or anteiso-C_{17 : 1}B), summed feature 3 (C_{16 : 1}ω6c and/or C_{16 : 1}ω7c), C_{17 : 1}ω6c, iso-C_{16 : 0}, iso-C_{15 : 0}and C_{16 : 1}ω5c. The predominant polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids, one glycolipid and four unidentified lipids. The G+C content of the genomic DNA was 49 mol%. On the basis of the phenotypic characteristics and phylogenetic analysis, strain MDT1-10-3^Trepresents a novel species of the genus Rufibacter, for which the name Rufibacter glacialis sp. nov. is proposed. The type strain is MDT1-10-3^T(=CGMCC 1.9789^T=NBRC 109705^T).

Original language	English
Article number	000717
Pages (from-to)	315-318
Number of pages	4
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	66
Issue number	1
DOIs	https://doi.org/10.1099/ijsem.0.000717
Publication status	Published - Jan 2016

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title = "Rufibacter glacialis sp. Nov., a psychrotolerant bacterium isolated from glacier soil",

abstract = "A Gram-stain-negative, rod-shaped, red-pigmented bacterium (MDT1-10-3T) was isolated from Midui glacier in Tibet, China. Cells were aerobic and psychrotolerant (growth occurred at 4-25 8C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that it was a member of the genus Rufibacter, with Rufibacter immobilis MCC P1T(96.7\% similarity) as its closest phylogenetic relative. MK-7 was the predominant respiratory menaquinone. The major cellular fatty acids were summed feature 4 (iso-C17 : 1I and/or anteiso-C17 : 1B), summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), C17 : 1ω6c, iso-C16 : 0, iso-C15 : 0and C16 : 1ω5c. The predominant polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids, one glycolipid and four unidentified lipids. The G+C content of the genomic DNA was 49 mol\%. On the basis of the phenotypic characteristics and phylogenetic analysis, strain MDT1-10-3Trepresents a novel species of the genus Rufibacter, for which the name Rufibacter glacialis sp. nov. is proposed. The type strain is MDT1-10-3T(=CGMCC 1.9789T=NBRC 109705T).",

author = "Qing Liu and Liu, \{Hong Can\} and Zhang, \{Jian Li\} and Zhou, \{Yu Guang\} and Xin, \{Yu Hua\}",

note = "Publisher Copyright: {\textcopyright} 2015 IUMS.",

year = "2016",

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doi = "10.1099/ijsem.0.000717",

language = "English",

volume = "66",

pages = "315--318",

journal = "International Journal of Systematic and Evolutionary Microbiology",

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AU - Liu, Qing

AU - Liu, Hong Can

AU - Zhang, Jian Li

AU - Zhou, Yu Guang

AU - Xin, Yu Hua

PY - 2016/1

Y1 - 2016/1

N2 - A Gram-stain-negative, rod-shaped, red-pigmented bacterium (MDT1-10-3T) was isolated from Midui glacier in Tibet, China. Cells were aerobic and psychrotolerant (growth occurred at 4-25 8C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that it was a member of the genus Rufibacter, with Rufibacter immobilis MCC P1T(96.7% similarity) as its closest phylogenetic relative. MK-7 was the predominant respiratory menaquinone. The major cellular fatty acids were summed feature 4 (iso-C17 : 1I and/or anteiso-C17 : 1B), summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), C17 : 1ω6c, iso-C16 : 0, iso-C15 : 0and C16 : 1ω5c. The predominant polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids, one glycolipid and four unidentified lipids. The G+C content of the genomic DNA was 49 mol%. On the basis of the phenotypic characteristics and phylogenetic analysis, strain MDT1-10-3Trepresents a novel species of the genus Rufibacter, for which the name Rufibacter glacialis sp. nov. is proposed. The type strain is MDT1-10-3T(=CGMCC 1.9789T=NBRC 109705T).

AB - A Gram-stain-negative, rod-shaped, red-pigmented bacterium (MDT1-10-3T) was isolated from Midui glacier in Tibet, China. Cells were aerobic and psychrotolerant (growth occurred at 4-25 8C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that it was a member of the genus Rufibacter, with Rufibacter immobilis MCC P1T(96.7% similarity) as its closest phylogenetic relative. MK-7 was the predominant respiratory menaquinone. The major cellular fatty acids were summed feature 4 (iso-C17 : 1I and/or anteiso-C17 : 1B), summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), C17 : 1ω6c, iso-C16 : 0, iso-C15 : 0and C16 : 1ω5c. The predominant polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids, one glycolipid and four unidentified lipids. The G+C content of the genomic DNA was 49 mol%. On the basis of the phenotypic characteristics and phylogenetic analysis, strain MDT1-10-3Trepresents a novel species of the genus Rufibacter, for which the name Rufibacter glacialis sp. nov. is proposed. The type strain is MDT1-10-3T(=CGMCC 1.9789T=NBRC 109705T).

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DO - 10.1099/ijsem.0.000717

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JF - International Journal of Systematic and Evolutionary Microbiology

IS - 1

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ER -

Rufibacter glacialis sp. Nov., a psychrotolerant bacterium isolated from glacier soil

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