Recombinant adenovirus vector-mediated functional expression of neurotropin-3 receptor (TrkC) in neural stem cells

Jun Mei Wang; Yuan Shan Zeng; Ran Yi Liu; Wen Lin Huang; Yi Xiong; Yan Hua Wang; Shui Jun Chen; Yang D. Teng

doi:10.1016/j.expneurol.2006.07.028

Recombinant adenovirus vector-mediated functional expression of neurotropin-3 receptor (TrkC) in neural stem cells

Jun Mei Wang
, Yuan Shan Zeng^*
, Ran Yi Liu
, Wen Lin Huang
, Yi Xiong
, Yan Hua Wang
, Shui Jun Chen
, Yang D. Teng

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

We have constructed a recombinant adenovirus expression vector carrying the human neurotrophin-3 (NT-3) receptor TrkC (tyrosine protein kinase C) gene (rAd-TrkC; 2478 bp) and confirmed the expression of the encoded TrkC in green fluorescent protein (GFP)-murine neural stem cells (NSCs) by reverse transcription polymerase chain reaction (RT-PCR), Western blot analysis, and immunocytochemistry. The activity of the expressed rAd-TrkC was verified in vitro by evaluating dose-related responses of NSCs to NT-3, a TrkC specific ligand. TrkC-GFP-NSCs had a significantly higher percentage of neuronal differentiation when treated with NT-3 relative to the rAd-LacZ control cells (55.2% vs. 29.8%; P < 0.05, χ² test). Thus, our rAd-TrkC vector can transfect NSCs and produce functional TrkC receptors to promote neuronal differentiation of NSCs.

Original language	English
Pages (from-to)	123-127
Number of pages	5
Journal	Experimental Neurology
Volume	203
Issue number	1
DOIs	https://doi.org/10.1016/j.expneurol.2006.07.028
Publication status	Published - Jan 2007
Externally published	Yes

Keywords

Differentiation
Gene expression
NT-3
Neural stem cell
Recombinant adenovirus
TrkC

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10.1016/j.expneurol.2006.07.028

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title = "Recombinant adenovirus vector-mediated functional expression of neurotropin-3 receptor (TrkC) in neural stem cells",

abstract = "We have constructed a recombinant adenovirus expression vector carrying the human neurotrophin-3 (NT-3) receptor TrkC (tyrosine protein kinase C) gene (rAd-TrkC; 2478 bp) and confirmed the expression of the encoded TrkC in green fluorescent protein (GFP)-murine neural stem cells (NSCs) by reverse transcription polymerase chain reaction (RT-PCR), Western blot analysis, and immunocytochemistry. The activity of the expressed rAd-TrkC was verified in vitro by evaluating dose-related responses of NSCs to NT-3, a TrkC specific ligand. TrkC-GFP-NSCs had a significantly higher percentage of neuronal differentiation when treated with NT-3 relative to the rAd-LacZ control cells (55.2\% vs. 29.8\%; P < 0.05, χ2 test). Thus, our rAd-TrkC vector can transfect NSCs and produce functional TrkC receptors to promote neuronal differentiation of NSCs.",

keywords = "Differentiation, Gene expression, NT-3, Neural stem cell, Recombinant adenovirus, TrkC",

author = "Wang, \{Jun Mei\} and Zeng, \{Yuan Shan\} and Liu, \{Ran Yi\} and Huang, \{Wen Lin\} and Yi Xiong and Wang, \{Yan Hua\} and Chen, \{Shui Jun\} and Teng, \{Yang D.\}",

year = "2007",

month = jan,

doi = "10.1016/j.expneurol.2006.07.028",

language = "English",

volume = "203",

pages = "123--127",

journal = "Experimental Neurology",

issn = "0014-4886",

publisher = "Academic Press Inc.",

number = "1",

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TY - JOUR

T1 - Recombinant adenovirus vector-mediated functional expression of neurotropin-3 receptor (TrkC) in neural stem cells

AU - Wang, Jun Mei

AU - Zeng, Yuan Shan

AU - Liu, Ran Yi

AU - Huang, Wen Lin

AU - Xiong, Yi

AU - Wang, Yan Hua

AU - Chen, Shui Jun

AU - Teng, Yang D.

PY - 2007/1

Y1 - 2007/1

N2 - We have constructed a recombinant adenovirus expression vector carrying the human neurotrophin-3 (NT-3) receptor TrkC (tyrosine protein kinase C) gene (rAd-TrkC; 2478 bp) and confirmed the expression of the encoded TrkC in green fluorescent protein (GFP)-murine neural stem cells (NSCs) by reverse transcription polymerase chain reaction (RT-PCR), Western blot analysis, and immunocytochemistry. The activity of the expressed rAd-TrkC was verified in vitro by evaluating dose-related responses of NSCs to NT-3, a TrkC specific ligand. TrkC-GFP-NSCs had a significantly higher percentage of neuronal differentiation when treated with NT-3 relative to the rAd-LacZ control cells (55.2% vs. 29.8%; P < 0.05, χ2 test). Thus, our rAd-TrkC vector can transfect NSCs and produce functional TrkC receptors to promote neuronal differentiation of NSCs.

AB - We have constructed a recombinant adenovirus expression vector carrying the human neurotrophin-3 (NT-3) receptor TrkC (tyrosine protein kinase C) gene (rAd-TrkC; 2478 bp) and confirmed the expression of the encoded TrkC in green fluorescent protein (GFP)-murine neural stem cells (NSCs) by reverse transcription polymerase chain reaction (RT-PCR), Western blot analysis, and immunocytochemistry. The activity of the expressed rAd-TrkC was verified in vitro by evaluating dose-related responses of NSCs to NT-3, a TrkC specific ligand. TrkC-GFP-NSCs had a significantly higher percentage of neuronal differentiation when treated with NT-3 relative to the rAd-LacZ control cells (55.2% vs. 29.8%; P < 0.05, χ2 test). Thus, our rAd-TrkC vector can transfect NSCs and produce functional TrkC receptors to promote neuronal differentiation of NSCs.

KW - Differentiation

KW - Gene expression

KW - NT-3

KW - Neural stem cell

KW - Recombinant adenovirus

KW - TrkC

UR - https://www.scopus.com/pages/publications/33751430281

U2 - 10.1016/j.expneurol.2006.07.028

DO - 10.1016/j.expneurol.2006.07.028

M3 - Article

C2 - 17007838

AN - SCOPUS:33751430281

SN - 0014-4886

VL - 203

SP - 123

EP - 127

JO - Experimental Neurology

JF - Experimental Neurology

IS - 1

ER -

Recombinant adenovirus vector-mediated functional expression of neurotropin-3 receptor (TrkC) in neural stem cells

Abstract

Keywords

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