TY - JOUR
T1 - Rapid and sensitive screening system of Mycoplasma hyorhinis based on Sandwich-DNA hybridization with nano-amplifiers
AU - Wang, Audrey
AU - Zhang, Xindan
AU - Shi, Haoran
AU - Lee, Youngkwan
AU - Jiang, Zhenqi
AU - Kim, Soochan
AU - Qin, Jieling
N1 - Publisher Copyright:
© 2023 The Authors
PY - 2023/12
Y1 - 2023/12
N2 - Mycoplasma contamination in cell/tissue cultures significantly affects cell characteristics, susceptibility to infectious pathogens, and drug reactions without obvious morphological changes and noticeable changes in cell growth rates. Although the technology for screening mycoplasma is essential, conventional technologies have limitations in the selection of a quick and easy manner. Here, we introduce an innovative screening system for mycoplasma based on electrochemical methods. In the electrochemical screening, a newly designed electrode, consisting of a sandwich-DNA hybridization for high selectivity and a nano-amplifier to improve the sensitivity, has implemented a realistic screening system by presenting detection capability at extremely low (ng/μL) levels and detection potential in real samples. Therefore, this system offers rapid screening, data accuracy, temporal effectiveness, and affordable-and-portable installation, enabling timely elimination of Mycoplasma contamination and facilitating progress in biomedical and pharmaceutical research.
AB - Mycoplasma contamination in cell/tissue cultures significantly affects cell characteristics, susceptibility to infectious pathogens, and drug reactions without obvious morphological changes and noticeable changes in cell growth rates. Although the technology for screening mycoplasma is essential, conventional technologies have limitations in the selection of a quick and easy manner. Here, we introduce an innovative screening system for mycoplasma based on electrochemical methods. In the electrochemical screening, a newly designed electrode, consisting of a sandwich-DNA hybridization for high selectivity and a nano-amplifier to improve the sensitivity, has implemented a realistic screening system by presenting detection capability at extremely low (ng/μL) levels and detection potential in real samples. Therefore, this system offers rapid screening, data accuracy, temporal effectiveness, and affordable-and-portable installation, enabling timely elimination of Mycoplasma contamination and facilitating progress in biomedical and pharmaceutical research.
KW - Early diagnosis
KW - Electrochemical biosensor
KW - Mycoplasma infection
KW - Plasmonic nano-amplifier
KW - Sandwich-DNA hybridization
UR - http://www.scopus.com/inward/record.url?scp=85175421843&partnerID=8YFLogxK
U2 - 10.1016/j.mtadv.2023.100437
DO - 10.1016/j.mtadv.2023.100437
M3 - Article
AN - SCOPUS:85175421843
SN - 2590-0498
VL - 20
JO - Materials Today Advances
JF - Materials Today Advances
M1 - 100437
ER -