Quantitative analysis of lymphocytes morphology and motion in intravital microscopic images

Yali Huang, Zhiwen Liu, Yonggang Shi, Ning Li, Xing An, Xiaoming Gou

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

9 Citations (Scopus)

Abstract

Studying the morphology and interior movement of lymphocytes in intravital microscopic images is essential to understanding and treating various biological processes and pathological situations. A method combing features of shape, deformation, and intracellular motion for quantitatively characterizing the dynamic behavior of a single lymphocyte is proposed in this paper. The method is tested on a set of image sequences of lymphocytes obtained from the peripheral blood of mice undergoing skin transplantation using a phase contrast microscope. Experimental results coincide with the clinical observation and pathological analysis, demonstrating that the extracted cell morphology and motion features can provide new insights into the relationship between the dynamic behavior of lymphocytes and the occurrence of graft rejection.

Original languageEnglish
Title of host publication2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
Pages3686-3689
Number of pages4
DOIs
Publication statusPublished - 2013
Event2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013 - Osaka, Japan
Duration: 3 Jul 20137 Jul 2013

Publication series

NameProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN (Print)1557-170X

Conference

Conference2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
Country/TerritoryJapan
CityOsaka
Period3/07/137/07/13

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