TY - JOUR
T1 - Quantitative analysis of atractylenolide I in rat plasma by LC-MS/MS method and its application to pharmacokinetic study
AU - Li, Yujuan
AU - Zhang, Yushi
AU - Wang, Zhimin
AU - Zhu, Jingjing
AU - Tian, Yuan
AU - Chen, Bo
PY - 2012/1/25
Y1 - 2012/1/25
N2 - A new high-performance liquid chromatography/tandem mass spectrometry (LC-MS/MS) was developed for quantitative analysis of atractylenolide I in rat plasma using buspirone as internal standard (I.S.). Rat plasma samples were deproteined with methanol and acetonitrile (1:1, v/v). Atractylenolide I and I.S. were separated on a Phenomenex Gemini C 18 column (50mm×2.0mm, 5μm) with gradient mobile phase at the flow rate of 0.4ml/min. The detection was performed by positive ion electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode. The linear calibration curve of atractylenolide I in rat plasma ranged 2.0-5000ng/ml (R>0.9979). The limit of detection (LOD) and the limit of quantification (LOQ) were 0.6ng/ml and 2.0ng/ml, respectively. Both accuracy and precision of the assay were satisfactory. The recoveries of atractylenolide I and I.S. were 91.4% and 87.8%, respectively. This fully validated method was applied to a pharmacokinetic study of atractylenolide I in rats administered with 20g/kg Atractylodis extract. The main pharmacokinetic parameters T max (the time to peak), C max (the concentration to peak), T 0.5 (the biological half time), and K e (the elimination rate constant) were 0.81±0.11h, 7.99±1.2ng/ml, 1.94±0.27h, 0.365±0.06/h, respectively.
AB - A new high-performance liquid chromatography/tandem mass spectrometry (LC-MS/MS) was developed for quantitative analysis of atractylenolide I in rat plasma using buspirone as internal standard (I.S.). Rat plasma samples were deproteined with methanol and acetonitrile (1:1, v/v). Atractylenolide I and I.S. were separated on a Phenomenex Gemini C 18 column (50mm×2.0mm, 5μm) with gradient mobile phase at the flow rate of 0.4ml/min. The detection was performed by positive ion electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode. The linear calibration curve of atractylenolide I in rat plasma ranged 2.0-5000ng/ml (R>0.9979). The limit of detection (LOD) and the limit of quantification (LOQ) were 0.6ng/ml and 2.0ng/ml, respectively. Both accuracy and precision of the assay were satisfactory. The recoveries of atractylenolide I and I.S. were 91.4% and 87.8%, respectively. This fully validated method was applied to a pharmacokinetic study of atractylenolide I in rats administered with 20g/kg Atractylodis extract. The main pharmacokinetic parameters T max (the time to peak), C max (the concentration to peak), T 0.5 (the biological half time), and K e (the elimination rate constant) were 0.81±0.11h, 7.99±1.2ng/ml, 1.94±0.27h, 0.365±0.06/h, respectively.
KW - Atractylenolide I
KW - Atractylodis
KW - LC-MS/MS
KW - Pharmacokientics
KW - Rat plasma
UR - http://www.scopus.com/inward/record.url?scp=80255140292&partnerID=8YFLogxK
U2 - 10.1016/j.jpba.2011.09.023
DO - 10.1016/j.jpba.2011.09.023
M3 - Article
C2 - 22014654
AN - SCOPUS:80255140292
SN - 0731-7085
VL - 58
SP - 172
EP - 176
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
IS - 1
ER -