TY - JOUR
T1 - Pulsed Direct Current Electrospray
T2 - Enabling Systematic Analysis of Small Volume Sample by Boosting Sample Economy
AU - Wei, Zhenwei
AU - Xiong, Xingchuang
AU - Guo, Chengan
AU - Si, Xingyu
AU - Zhao, Yaoyao
AU - He, Muyi
AU - Yang, Chengdui
AU - Xu, Wei
AU - Tang, Fei
AU - Fang, Xiang
AU - Zhang, Sichun
AU - Zhang, Xinrong
N1 - Publisher Copyright:
© 2015 American Chemical Society.
PY - 2015/10/21
Y1 - 2015/10/21
N2 - We had developed pulsed direct current electrospray ionization mass spectrometry (pulsed-dc-ESI-MS) for systematically profiling and determining components in small volume sample. Pulsed-dc-ESI utilized constant high voltage to induce the generation of single polarity pulsed electrospray remotely. This method had significantly boosted the sample economy, so as to obtain several minutes MS signal duration from merely picoliter volume sample. The elongated MS signal duration enable us to collect abundant MS2 information on interested components in a small volume sample for systematical analysis. This method had been successfully applied for single cell metabolomics analysis. We had obtained 2-D profile of metabolites (including exact mass and MS2 data) from single plant and mammalian cell, concerning 1034 components and 656 components for Allium cepa and HeLa cells, respectively. Further identification had found 162 compounds and 28 different modification groups of 141 saccharides in a single Allium cepa cell, indicating pulsed-dc-ESI a powerful tool for small volume sample systematical analysis.
AB - We had developed pulsed direct current electrospray ionization mass spectrometry (pulsed-dc-ESI-MS) for systematically profiling and determining components in small volume sample. Pulsed-dc-ESI utilized constant high voltage to induce the generation of single polarity pulsed electrospray remotely. This method had significantly boosted the sample economy, so as to obtain several minutes MS signal duration from merely picoliter volume sample. The elongated MS signal duration enable us to collect abundant MS2 information on interested components in a small volume sample for systematical analysis. This method had been successfully applied for single cell metabolomics analysis. We had obtained 2-D profile of metabolites (including exact mass and MS2 data) from single plant and mammalian cell, concerning 1034 components and 656 components for Allium cepa and HeLa cells, respectively. Further identification had found 162 compounds and 28 different modification groups of 141 saccharides in a single Allium cepa cell, indicating pulsed-dc-ESI a powerful tool for small volume sample systematical analysis.
UR - http://www.scopus.com/inward/record.url?scp=84947223456&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.5b02115
DO - 10.1021/acs.analchem.5b02115
M3 - Article
C2 - 26488206
AN - SCOPUS:84947223456
SN - 0003-2700
VL - 87
SP - 11242
EP - 11248
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 22
ER -