Preliminary screening of peptide biomarker for early diagnosis of diabetes

Mei Zhang; Ke Ming Shan; Yu Lin Deng

doi:10.15918/j.tbit1001-0645.2015.supplement1.026

Preliminary screening of peptide biomarker for early diagnosis of diabetes

Mei Zhang^*
, Ke Ming Shan
, Yu Lin Deng

^*Corresponding author for this work

School of Medical Science and Engineering

Chinese Center for Disease Control and Prevention
State Key Laboratory for Infectious Disease Prevention and Control
Zhejiang University
Norinco Group

Research output: Contribution to journal › Article › peer-review

Abstract

An in vitro glycation model was set up by glucose and human serum albumin (HSA) based on Maillard Reaction. HPLC-ESI TOF MS and ¹⁸O-labeling technique were employed to quantify glycation level of peptides cleaved from HSA. In order to screen glucose-sensitive peptides for being diabetic biomarker-candidates, the in vitro glycation model was set up and confirmed, and eleven biomarker-candidates were screened based on the model by HPLC-ESI TOF MS quantification. Eleven glucose-sensitive peptides were screened for being biomarker-candidates, which needs to be verified in clinical trials in the future.

Original language	English
Pages (from-to)	119-122
Number of pages	4
Journal	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
Volume	35
DOIs	https://doi.org/10.15918/j.tbit1001-0645.2015.supplement1.026
Publication status	Published - 1 Jul 2015

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Biomarker
Diabetes
HPLC-ESI TOF MS
Human serum albumin
O-labeling

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10.15918/j.tbit1001-0645.2015.supplement1.026

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title = "Preliminary screening of peptide biomarker for early diagnosis of diabetes",

abstract = "An in vitro glycation model was set up by glucose and human serum albumin (HSA) based on Maillard Reaction. HPLC-ESI TOF MS and 18O-labeling technique were employed to quantify glycation level of peptides cleaved from HSA. In order to screen glucose-sensitive peptides for being diabetic biomarker-candidates, the in vitro glycation model was set up and confirmed, and eleven biomarker-candidates were screened based on the model by HPLC-ESI TOF MS quantification. Eleven glucose-sensitive peptides were screened for being biomarker-candidates, which needs to be verified in clinical trials in the future.",

keywords = "Biomarker, Diabetes, HPLC-ESI TOF MS, Human serum albumin, O-labeling",

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AU - Zhang, Mei

AU - Shan, Ke Ming

AU - Deng, Yu Lin

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AB - An in vitro glycation model was set up by glucose and human serum albumin (HSA) based on Maillard Reaction. HPLC-ESI TOF MS and 18O-labeling technique were employed to quantify glycation level of peptides cleaved from HSA. In order to screen glucose-sensitive peptides for being diabetic biomarker-candidates, the in vitro glycation model was set up and confirmed, and eleven biomarker-candidates were screened based on the model by HPLC-ESI TOF MS quantification. Eleven glucose-sensitive peptides were screened for being biomarker-candidates, which needs to be verified in clinical trials in the future.

KW - Biomarker

KW - Diabetes

KW - HPLC-ESI TOF MS

KW - Human serum albumin

KW - O-labeling

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M3 - Article

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SN - 1001-0645

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SP - 119

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JO - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

ER -

Preliminary screening of peptide biomarker for early diagnosis of diabetes

Abstract

UN SDGs

Keywords

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