Preliminary screening of peptide biomarker for early diagnosis of diabetes

Mei Zhang; Ke Ming Shan; Yu Lin Deng

doi:10.15918/j.tbit1001-0645.2015.supplement1.026

Preliminary screening of peptide biomarker for early diagnosis of diabetes

Mei Zhang^*
, Ke Ming Shan
, Yu Lin Deng

^*Corresponding author for this work

School of Medical Technology

Research output: Contribution to journal › Article › peer-review

Abstract

An in vitro glycation model was set up by glucose and human serum albumin (HSA) based on Maillard Reaction. HPLC-ESI TOF MS and ¹⁸O-labeling technique were employed to quantify glycation level of peptides cleaved from HSA. In order to screen glucose-sensitive peptides for being diabetic biomarker-candidates, the in vitro glycation model was set up and confirmed, and eleven biomarker-candidates were screened based on the model by HPLC-ESI TOF MS quantification. Eleven glucose-sensitive peptides were screened for being biomarker-candidates, which needs to be verified in clinical trials in the future.

Original language	English
Pages (from-to)	119-122
Number of pages	4
Journal	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
Volume	35
DOIs	https://doi.org/10.15918/j.tbit1001-0645.2015.supplement1.026
Publication status	Published - 1 Jul 2015

Keywords

Biomarker
Diabetes
HPLC-ESI TOF MS
Human serum albumin
O-labeling

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.15918/j.tbit1001-0645.2015.supplement1.026

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title = "Preliminary screening of peptide biomarker for early diagnosis of diabetes",

abstract = "An in vitro glycation model was set up by glucose and human serum albumin (HSA) based on Maillard Reaction. HPLC-ESI TOF MS and 18O-labeling technique were employed to quantify glycation level of peptides cleaved from HSA. In order to screen glucose-sensitive peptides for being diabetic biomarker-candidates, the in vitro glycation model was set up and confirmed, and eleven biomarker-candidates were screened based on the model by HPLC-ESI TOF MS quantification. Eleven glucose-sensitive peptides were screened for being biomarker-candidates, which needs to be verified in clinical trials in the future.",

keywords = "Biomarker, Diabetes, HPLC-ESI TOF MS, Human serum albumin, O-labeling",

author = "Mei Zhang and Shan, \{Ke Ming\} and Deng, \{Yu Lin\}",

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doi = "10.15918/j.tbit1001-0645.2015.supplement1.026",

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T1 - Preliminary screening of peptide biomarker for early diagnosis of diabetes

AU - Zhang, Mei

AU - Shan, Ke Ming

AU - Deng, Yu Lin

PY - 2015/7/1

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N2 - An in vitro glycation model was set up by glucose and human serum albumin (HSA) based on Maillard Reaction. HPLC-ESI TOF MS and 18O-labeling technique were employed to quantify glycation level of peptides cleaved from HSA. In order to screen glucose-sensitive peptides for being diabetic biomarker-candidates, the in vitro glycation model was set up and confirmed, and eleven biomarker-candidates were screened based on the model by HPLC-ESI TOF MS quantification. Eleven glucose-sensitive peptides were screened for being biomarker-candidates, which needs to be verified in clinical trials in the future.

AB - An in vitro glycation model was set up by glucose and human serum albumin (HSA) based on Maillard Reaction. HPLC-ESI TOF MS and 18O-labeling technique were employed to quantify glycation level of peptides cleaved from HSA. In order to screen glucose-sensitive peptides for being diabetic biomarker-candidates, the in vitro glycation model was set up and confirmed, and eleven biomarker-candidates were screened based on the model by HPLC-ESI TOF MS quantification. Eleven glucose-sensitive peptides were screened for being biomarker-candidates, which needs to be verified in clinical trials in the future.

KW - Biomarker

KW - Diabetes

KW - HPLC-ESI TOF MS

KW - Human serum albumin

KW - O-labeling

UR - https://www.scopus.com/pages/publications/84943424135

U2 - 10.15918/j.tbit1001-0645.2015.supplement1.026

DO - 10.15918/j.tbit1001-0645.2015.supplement1.026

M3 - Article

AN - SCOPUS:84943424135

SN - 1001-0645

VL - 35

SP - 119

EP - 122

JO - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

ER -

Preliminary screening of peptide biomarker for early diagnosis of diabetes

Abstract

Keywords

UN SDGs

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