Potential toxicity of chronic creatine supplementation in mice

Lin Wang; Shengyuan Xiao; Yujuan Li; Lu Wang; Baoquan Che; Xuan Zhao; Yulin Deng

doi:10.1109/ICBBE.2009.5163209

Potential toxicity of chronic creatine supplementation in mice

Lin Wang
, Shengyuan Xiao
, Yujuan Li
, Lu Wang
, Baoquan Che
, Xuan Zhao
, Yulin Deng^*

^*Corresponding author for this work

Beijing Institute of Technology
Beijing Institute for Drug Control

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Citations (Scopus)

Abstract

Creatine is alleged to be a popular nutrition to enhance sports performance. It can be metabolized to methylamine, which is further converted to formaldehyde, hydrogen peroxide and ammonium by semicarbazied-sensitive amine oxidases (SSAO). Until now, there is no scientific available data for demonstration the long-term health risks of chronic creatine ingestion. In this study, we demonstrated that after chronic oral administration of creatine to mice, the level of methylamine and formaldehyde in urine were increased, SSAO activity of serum and tissue were increased simultaneously. SSAO inhibitor (2-bromoethylamine hydrobromide) could substantially increase the methylamine level and decrease the formaldehyde content respectively. A novel high-performance liquid chromatography (HPLC) and electrospray ionization (ESI) mass spectrometry method for the determination of SSAO activity was developed for the first time. Potential toxicity after chronic creatine administration was discussed according to the increase of toxic products content and SSAO activity. To our knowledge, this is the first report on the research of relationship between SSAO and creatine.

Original language	English
Title of host publication	3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
DOIs	https://doi.org/10.1109/ICBBE.2009.5163209
Publication status	Published - 2009
Event	3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009 - Beijing, China Duration: 11 Jun 2009 → 13 Jun 2009

Publication series

Name	3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009

Conference

Conference	3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
Country/Territory	China
City	Beijing
Period	11/06/09 → 13/06/09

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Creatine
Formaldehyde
HPLC-ESI-MS
Methylamine
SSAO
Toxicity

Access to Document

10.1109/ICBBE.2009.5163209

Cite this

Wang, L., Xiao, S., Li, Y., Wang, L., Che, B., Zhao, X., & Deng, Y. (2009). Potential toxicity of chronic creatine supplementation in mice. In 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009 Article 5163209 (3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009). https://doi.org/10.1109/ICBBE.2009.5163209

@inproceedings{60378d91b136444699e58d7cbe84b37a,

title = "Potential toxicity of chronic creatine supplementation in mice",

abstract = "Creatine is alleged to be a popular nutrition to enhance sports performance. It can be metabolized to methylamine, which is further converted to formaldehyde, hydrogen peroxide and ammonium by semicarbazied-sensitive amine oxidases (SSAO). Until now, there is no scientific available data for demonstration the long-term health risks of chronic creatine ingestion. In this study, we demonstrated that after chronic oral administration of creatine to mice, the level of methylamine and formaldehyde in urine were increased, SSAO activity of serum and tissue were increased simultaneously. SSAO inhibitor (2-bromoethylamine hydrobromide) could substantially increase the methylamine level and decrease the formaldehyde content respectively. A novel high-performance liquid chromatography (HPLC) and electrospray ionization (ESI) mass spectrometry method for the determination of SSAO activity was developed for the first time. Potential toxicity after chronic creatine administration was discussed according to the increase of toxic products content and SSAO activity. To our knowledge, this is the first report on the research of relationship between SSAO and creatine.",

keywords = "Creatine, Formaldehyde, HPLC-ESI-MS, Methylamine, SSAO, Toxicity",

author = "Lin Wang and Shengyuan Xiao and Yujuan Li and Lu Wang and Baoquan Che and Xuan Zhao and Yulin Deng",

year = "2009",

doi = "10.1109/ICBBE.2009.5163209",

language = "English",

isbn = "9781424429028",

series = "3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009",

booktitle = "3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009",

note = "3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009 ; Conference date: 11-06-2009 Through 13-06-2009",

}

Wang, L, Xiao, S, Li, Y, Wang, L, Che, B, Zhao, X & Deng, Y 2009, Potential toxicity of chronic creatine supplementation in mice. in 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009., 5163209, 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009, 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009, Beijing, China, 11/06/09. https://doi.org/10.1109/ICBBE.2009.5163209

Potential toxicity of chronic creatine supplementation in mice. / Wang, Lin; Xiao, Shengyuan; Li, Yujuan et al.
3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009. 2009. 5163209 (3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Potential toxicity of chronic creatine supplementation in mice

AU - Wang, Lin

AU - Xiao, Shengyuan

AU - Li, Yujuan

AU - Wang, Lu

AU - Che, Baoquan

AU - Zhao, Xuan

AU - Deng, Yulin

PY - 2009

Y1 - 2009

N2 - Creatine is alleged to be a popular nutrition to enhance sports performance. It can be metabolized to methylamine, which is further converted to formaldehyde, hydrogen peroxide and ammonium by semicarbazied-sensitive amine oxidases (SSAO). Until now, there is no scientific available data for demonstration the long-term health risks of chronic creatine ingestion. In this study, we demonstrated that after chronic oral administration of creatine to mice, the level of methylamine and formaldehyde in urine were increased, SSAO activity of serum and tissue were increased simultaneously. SSAO inhibitor (2-bromoethylamine hydrobromide) could substantially increase the methylamine level and decrease the formaldehyde content respectively. A novel high-performance liquid chromatography (HPLC) and electrospray ionization (ESI) mass spectrometry method for the determination of SSAO activity was developed for the first time. Potential toxicity after chronic creatine administration was discussed according to the increase of toxic products content and SSAO activity. To our knowledge, this is the first report on the research of relationship between SSAO and creatine.

AB - Creatine is alleged to be a popular nutrition to enhance sports performance. It can be metabolized to methylamine, which is further converted to formaldehyde, hydrogen peroxide and ammonium by semicarbazied-sensitive amine oxidases (SSAO). Until now, there is no scientific available data for demonstration the long-term health risks of chronic creatine ingestion. In this study, we demonstrated that after chronic oral administration of creatine to mice, the level of methylamine and formaldehyde in urine were increased, SSAO activity of serum and tissue were increased simultaneously. SSAO inhibitor (2-bromoethylamine hydrobromide) could substantially increase the methylamine level and decrease the formaldehyde content respectively. A novel high-performance liquid chromatography (HPLC) and electrospray ionization (ESI) mass spectrometry method for the determination of SSAO activity was developed for the first time. Potential toxicity after chronic creatine administration was discussed according to the increase of toxic products content and SSAO activity. To our knowledge, this is the first report on the research of relationship between SSAO and creatine.

KW - Creatine

KW - Formaldehyde

KW - HPLC-ESI-MS

KW - Methylamine

KW - SSAO

KW - Toxicity

UR - https://www.scopus.com/pages/publications/72749092036

U2 - 10.1109/ICBBE.2009.5163209

DO - 10.1109/ICBBE.2009.5163209

M3 - Conference contribution

AN - SCOPUS:72749092036

SN - 9781424429028

T3 - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009

BT - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009

T2 - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009

Y2 - 11 June 2009 through 13 June 2009

ER -

Potential toxicity of chronic creatine supplementation in mice

Abstract

Publication series

Conference

UN SDGs

Keywords

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