Pharmacokinetic study and tissue distribution of single mangiferin after intravenous administration in rats

A simple, sensitive ane selective high performance liquid chromatographic (HPLC) method with UV detection (320 nm) was developed and validated for determination of mangiferin in rat plasma and tissues. Mangiferin and internal standard (spinosin) were separated using mobile phase of acetonitrile-water (20:80, v/v) with 1% glacial acetic acid and 1% THF on a Phenomenex gemini C₁₈ column. The flow rate was 0.7 mL/min. The calibration curves of mangiferin in plasma and tissues were linear over the investigated ranges. The intra-and inter-run precisions for all samples were less than 13.8%. The time-concentration curve of mangiferin after intravenous administration to rats corresponded to two-compartment model. The main pharmacokinetic parameters T_0.5α, T_0.5β, CL and AUC_0-T were 15.87 min, 26.15 min, 6.1 L/(min·kg) and 3.28 mg·min/mL, respectively. The highest and lowest levels of mangiferin occurred in spleen and brain, respectively. Mangiferin was not found in liver. After intravenous administration, the drug was distributed extensively and transferred quickly in rats in vivo.