TY - JOUR
T1 - Pathogenicity of a Vibrio owensii strain isolated from Fenneropenaeus chinensis carrying pirAB genes and causing AHPND
AU - Liu, Fei
AU - Li, Shihao
AU - Yu, Yang
AU - Yuan, Jianbo
AU - Yu, Kuijie
AU - Li, Fuhua
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/1/15
Y1 - 2021/1/15
N2 - An emergent shrimp disease, acute hepatopancreatic necrosis disease (AHPND), has been causing great economic losses to the shrimp farming industry. The original causing agent of AHPND was regarded as Vibrio parahaemolyticus, with a plasmid named pVA1 carrying two Photorhabdus insect-related (Pir) toxin genes pirA and pirB. In the present study, a Vibrio owensii strain named FcYS03 was isolated from the Chinese shrimp Fenneropenaeus chinensis, which caused a high mortality in shrimp with typical AHPND signs. This strain carried the binary toxin genes pirAB and could cause AHPND both in Fenneropenaeus chinensis and Penaeus vannamei (previously named Litopenaeus vannamei). The pathogenicity of FcYS03 to P. vannamei exhibited apparent temperature and salinity dependence. Through genome sequence analysis among FcYS03 and AHPND-causing V. parahaemolyticus strain 3HP, we found that a segmental rearrangement phenomenon existed in the plasmid of FcYS03. The toxin genes pirAB happened to be located at the rearranged segment, and two transposases for transposon Tn903 were distributed at the two ends of the rearranged segment, which indicated that transposon Tn903 might be related to the transfer of the toxin genes among the bacteria. The study will provide valuable information for the understanding and prevention of AHPND in shrimp aquaculture.
AB - An emergent shrimp disease, acute hepatopancreatic necrosis disease (AHPND), has been causing great economic losses to the shrimp farming industry. The original causing agent of AHPND was regarded as Vibrio parahaemolyticus, with a plasmid named pVA1 carrying two Photorhabdus insect-related (Pir) toxin genes pirA and pirB. In the present study, a Vibrio owensii strain named FcYS03 was isolated from the Chinese shrimp Fenneropenaeus chinensis, which caused a high mortality in shrimp with typical AHPND signs. This strain carried the binary toxin genes pirAB and could cause AHPND both in Fenneropenaeus chinensis and Penaeus vannamei (previously named Litopenaeus vannamei). The pathogenicity of FcYS03 to P. vannamei exhibited apparent temperature and salinity dependence. Through genome sequence analysis among FcYS03 and AHPND-causing V. parahaemolyticus strain 3HP, we found that a segmental rearrangement phenomenon existed in the plasmid of FcYS03. The toxin genes pirAB happened to be located at the rearranged segment, and two transposases for transposon Tn903 were distributed at the two ends of the rearranged segment, which indicated that transposon Tn903 might be related to the transfer of the toxin genes among the bacteria. The study will provide valuable information for the understanding and prevention of AHPND in shrimp aquaculture.
KW - AHPND
KW - Environmental factor
KW - Fenneropenaeus chinensis
KW - Vibrio owensii
UR - http://www.scopus.com/inward/record.url?scp=85088856046&partnerID=8YFLogxK
U2 - 10.1016/j.aquaculture.2020.735747
DO - 10.1016/j.aquaculture.2020.735747
M3 - Article
AN - SCOPUS:85088856046
SN - 0044-8486
VL - 530
JO - Aquaculture
JF - Aquaculture
M1 - 735747
ER -