Optimized luteolin loaded solid lipid nanoparticle under stress condition for enhanced bioavailability in rat plasma

Luteolin (LU, 5,7,3',4'-tetrahydroxyflavone) has antimicrobial, anti-inflammatory, anti-cancer, and antimutagen activities but has limited application due to hydrophobic nature. In the present study, Luteolin (LU, 5,7,3',4'-tetrahydroxyflavone) was successfully loaded in solid lipid nanoparticles by hot homogenious mixture-sonication method. Luteolin with loaded solid lipid nanopartcile showed better pharmacokinetics as compared to pure Luteolin. Transmission Electron Microscope result showed round shaped luteolin loaded solid lipid nanoparticles with size 61.80 nm, Zeta potential -8.91 mV, and drug entrapment efficiency 74.11%. Furthermore, Luteolin loaded solid lipid nanoparticle showed the stability against the different pH as well as temperature. Validity of method was tested and Less than 2% RSD value indicates the validity of method in uptake of letolin loaded with solid lipid nanoparticles in rat plasma as compared to naked drug. XRD analysis also supports the use of solid lipid nanoparticle as drug delivery. Finally, the different parameters were optimized to attain the stability for more than two months without changing physio-chemical characteristic of solid lipid nanoparticles. Pharmacokinetics studies also support the loading of Luteolin in solid lipid nanoparticle as drug delivery. In conclusion, we can say that solid lipid nanoparticle provides promising platform hydrophobic drugs like luteolin. In the future, these solid nanoparticles can be used as multifunctional unit as cargo in the field of biomedical technology.