TY - JOUR
T1 - Nocardioides glacieisoli sp. Nov, isolated from a glacier
AU - Liu, Qing
AU - Liu, Hong Can
AU - Zhang, Jian Li
AU - Zhou, Yu Guang
AU - Xin, Yu Hua
AU - Xin, Yu Hua
N1 - Publisher Copyright:
© 2015 IUMS.
PY - 2015/12
Y1 - 2015/12
N2 - A Gram-stain-positive, rod-shaped, non-spore-forming bacterium (strain HLT3-15T) was isolated from the ice tongue surface of the Hailuogou glacier in Szechwan Province, PR China. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain HLT3-15T belonged to the genus Nocardioides. The highest levels of sequence similarities were found with Nocardioides hwasunensis CGMCC 4.6881T and Nocardioides ganghwensis CGMCC 4.6875T (98.5 % and 98.3 %, respectively). However, DNA–DNA relatedness demonstrated that strain HLT3-15T was distinct from its closest phylogenetic neighbours. The major cellular fatty acids of strain HLT3-15T were C17 : 1ω8c and iso-C16 : 0. Strain HLT3-15T contained LL-2,6-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan and MK-8(H4) as the predominant menaquinone. On the basis of a polyphasic approach, a novel species, Nocardioides glacieisoli sp. Nov, is proposed with HLT3-15T (=CGMCC 1.11097T =NBRC 109781T) as the type strain.
AB - A Gram-stain-positive, rod-shaped, non-spore-forming bacterium (strain HLT3-15T) was isolated from the ice tongue surface of the Hailuogou glacier in Szechwan Province, PR China. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain HLT3-15T belonged to the genus Nocardioides. The highest levels of sequence similarities were found with Nocardioides hwasunensis CGMCC 4.6881T and Nocardioides ganghwensis CGMCC 4.6875T (98.5 % and 98.3 %, respectively). However, DNA–DNA relatedness demonstrated that strain HLT3-15T was distinct from its closest phylogenetic neighbours. The major cellular fatty acids of strain HLT3-15T were C17 : 1ω8c and iso-C16 : 0. Strain HLT3-15T contained LL-2,6-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan and MK-8(H4) as the predominant menaquinone. On the basis of a polyphasic approach, a novel species, Nocardioides glacieisoli sp. Nov, is proposed with HLT3-15T (=CGMCC 1.11097T =NBRC 109781T) as the type strain.
UR - http://www.scopus.com/inward/record.url?scp=84955482681&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.000658
DO - 10.1099/ijsem.0.000658
M3 - Article
C2 - 26442516
AN - SCOPUS:84955482681
SN - 1466-5026
VL - 65
SP - 4845
EP - 4849
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 12
ER -