Abstract
The traditional MTT assay requires destructive analyses and is not convenient in continuous monitory of cell viability. However, a new cell model was developed in this research, by using the oxidation-reduction (redox) indicator alamarblue™, instead of the MTT assay. The alamarblue™ does no harm to cells and provide a much more safe and convenient methods of measurement. Firstly, cell apoptosis was induced by different concentration of 6-OHDA, then the cell viability was tested by the alamarblue™ at a serial of time points. Finally, the optimism cells density, 6-OHDA concentration and testing time point were gained to set a wonderful SH-SY5Y cell model in our research. And when it is applied in the study of neuroprotection effects of NAC, GSH and Catalase, the new model reveals undeniable advantages.
| Original language | English |
|---|---|
| Pages (from-to) | 98-103 |
| Number of pages | 6 |
| Journal | Journal of Beijing Institute of Technology (English Edition) |
| Volume | 13 |
| Issue number | SUPPL. |
| Publication status | Published - Dec 2004 |
Keywords
- Alamarblue
- Cell model
- PD research
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