TY - JOUR
T1 - Near-Infrared Probe Based on Rhodamine Derivative for Highly Sensitive and Selective Lysosomal pH Tracking
AU - Niu, Guangle
AU - Zhang, Panpan
AU - Liu, Weimin
AU - Wang, Mengqi
AU - Zhang, Hongyan
AU - Wu, Jiasheng
AU - Zhang, Liping
AU - Wang, Pengfei
N1 - Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/2/7
Y1 - 2017/2/7
N2 - The development of near-infrared fluorescent probes with low pKa, high selectivity, high photostability, and high sensitivity for lysosomal pH detection is of great importance. In the present work, we developed a novel near-infrared lysosomal pH probe (Lyso-hNR) based on a rhodamine derivative. Lyso-hNR showed fast, highly sensitive, and highly selective fluorescence response to acidic pH caused by the H+-induced structure changes from the nonfluorescent spirolactam form to the highly emissive open-ring form. Lyso-hNR displays a significant fluorescence enhancement at 650 nm (over 280-fold) from pH 7.0 to 4.0 with a pKa value of 5.04. Live cell imaging data revealed that Lyso-hNR can selectively monitor lysosomal pH changes with excellent photostability and low cytotoxicity. In addition, Lyso-hNR can be successfully used in tracking lysosomal pH changes induced by chloroquine and those during apoptosis. All these features render Lyso-hNR a promising candidate to investigate lysosome-associated physiological and pathological processes.
AB - The development of near-infrared fluorescent probes with low pKa, high selectivity, high photostability, and high sensitivity for lysosomal pH detection is of great importance. In the present work, we developed a novel near-infrared lysosomal pH probe (Lyso-hNR) based on a rhodamine derivative. Lyso-hNR showed fast, highly sensitive, and highly selective fluorescence response to acidic pH caused by the H+-induced structure changes from the nonfluorescent spirolactam form to the highly emissive open-ring form. Lyso-hNR displays a significant fluorescence enhancement at 650 nm (over 280-fold) from pH 7.0 to 4.0 with a pKa value of 5.04. Live cell imaging data revealed that Lyso-hNR can selectively monitor lysosomal pH changes with excellent photostability and low cytotoxicity. In addition, Lyso-hNR can be successfully used in tracking lysosomal pH changes induced by chloroquine and those during apoptosis. All these features render Lyso-hNR a promising candidate to investigate lysosome-associated physiological and pathological processes.
UR - http://www.scopus.com/inward/record.url?scp=85026767626&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.6b04417
DO - 10.1021/acs.analchem.6b04417
M3 - Article
C2 - 28208300
AN - SCOPUS:85026767626
SN - 0003-2700
VL - 89
SP - 1922
EP - 1929
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 3
ER -