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Nanopost Array Integrated Digital Microfluidics for Exosome Enrichment and Molecular Ionization

  • Liyuan Guo
  • , Menglei Zhao
  • , Boyu Li
  • , Bin Fu
  • , Rongxin Fu
  • , Kangfu Chen
  • , Hang Li*
  • , Shuailong Zhang*
  • *Corresponding author for this work
  • Beijing Institute of Technology
  • National Center for Protein Sciences (Beijing)

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

This study presents a nanopost array-embedded digital microfluidic (DMF) platform combined with laser desorption ionization (LDI) mass spectrometry (MS) for the enrichment and direct ionization of exosomes. The nanopost array, surface-modified with functional antibodies, is embedded in the top substrate of the DMF chip, enabling the automated isolation of exosomes from samples in low volumes (<2 μ L). The enriched exosomes on the nanoposts are then subjected to laser pulses, which resonate with the array to produce ionized exosomal molecules. The established platform has achieved a limit of detection (LOD) of 10-7 mol/ L for verapamil. This method allows for direct molecular analysis of DMF-processed samples by MS, offering an efficient and streamlined approach for bioclinical applications, particularly in exosome analysis.

Original languageEnglish
Title of host publication2026 IEEE 39th International Conference on Micro Electro Mechanical Systems, MEMS 2026
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1587-1590
Number of pages4
ISBN (Electronic)9798331572518
DOIs
Publication statusPublished - 2026
Event39th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2026 - Salzburg, Austria
Duration: 25 Jan 202629 Jan 2026

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Conference

Conference39th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2026
Country/TerritoryAustria
CitySalzburg
Period25/01/2629/01/26

Keywords

  • Digital Microfluidics
  • Exosome
  • Mass Spectrometry
  • Nanopost Array

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