Multifunctional Nanoprobe for 3D Nanoresolution Imaging of Intact Cell HER2 Protein with Hard X-ray Tomography

Chunyu Zhang; Kai Zhang; Yanyan Cui; Yuecong Guo; Chuan Wang; Chao Xu; Qingqiang Yao; Yuliang Zhao; Chunying Chen; Yaling Wang

doi:10.1021/acs.analchem.2c03699

Multifunctional Nanoprobe for 3D Nanoresolution Imaging of Intact Cell HER2 Protein with Hard X-ray Tomography

Chunyu Zhang
, Kai Zhang
, Yanyan Cui
, Yuecong Guo
, Chuan Wang
, Chao Xu
, Qingqiang Yao
, Yuliang Zhao
, Chunying Chen^*
, Yaling Wang^*

^*Corresponding author for this work

Shandong First Medical University & Shandong Academy of Medical Sciences
CAS - Institute of High Energy Physics
Beijing Institute of Technology
National Center for Nanoscience and Technology
The GBA National Institute for Nanotechnology Innovation
Shandong Agricultural University

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Three-dimensional nondestructive, nanoresolution, and in situ visualization of protein spatial localization in a large, thick single cell remains challenging. In this study, we designed a multifunctional iron oxide (Fe@BFK) nanoprobe that possesses fluorescence and hard X-ray imaging signals. This probe can specifically target the human epidermal growth factor receptor 2 (HER2) protein and help optimize the label condition and selection of suitable samples for X-ray imaging. Combining 30 nm resolution synchrotron radiation hard X-ray nanocomputed tomography and the X-ray-sensitive Fe@BFK nanoprobe, a 3D localization of HER2 on SK-BR-3 cells was obtained for the first time. HER2 was mainly localized and cluster-distributed on the cell membrane with a heterogeneous pattern. This study provides a novel method for the in situ and nondestructive synchrotron radiation imaging of the desired protein localization in large, thick cells and evaluation of the true cellular distribution of a nanoprobe with high resolution.

Original language	English
Pages (from-to)	2129-2133
Number of pages	5
Journal	Analytical Chemistry
Volume	95
Issue number	4
DOIs	https://doi.org/10.1021/acs.analchem.2c03699
Publication status	Published - 31 Jan 2023
Externally published	Yes

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10.1021/acs.analchem.2c03699

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title = "Multifunctional Nanoprobe for 3D Nanoresolution Imaging of Intact Cell HER2 Protein with Hard X-ray Tomography",

abstract = "Three-dimensional nondestructive, nanoresolution, and in situ visualization of protein spatial localization in a large, thick single cell remains challenging. In this study, we designed a multifunctional iron oxide (Fe@BFK) nanoprobe that possesses fluorescence and hard X-ray imaging signals. This probe can specifically target the human epidermal growth factor receptor 2 (HER2) protein and help optimize the label condition and selection of suitable samples for X-ray imaging. Combining 30 nm resolution synchrotron radiation hard X-ray nanocomputed tomography and the X-ray-sensitive Fe@BFK nanoprobe, a 3D localization of HER2 on SK-BR-3 cells was obtained for the first time. HER2 was mainly localized and cluster-distributed on the cell membrane with a heterogeneous pattern. This study provides a novel method for the in situ and nondestructive synchrotron radiation imaging of the desired protein localization in large, thick cells and evaluation of the true cellular distribution of a nanoprobe with high resolution.",

author = "Chunyu Zhang and Kai Zhang and Yanyan Cui and Yuecong Guo and Chuan Wang and Chao Xu and Qingqiang Yao and Yuliang Zhao and Chunying Chen and Yaling Wang",

note = "Publisher Copyright: {\textcopyright} 2022 American Chemical Society.",

year = "2023",

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day = "31",

doi = "10.1021/acs.analchem.2c03699",

language = "English",

volume = "95",

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journal = "Analytical Chemistry",

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T1 - Multifunctional Nanoprobe for 3D Nanoresolution Imaging of Intact Cell HER2 Protein with Hard X-ray Tomography

AU - Zhang, Chunyu

AU - Zhang, Kai

AU - Cui, Yanyan

AU - Guo, Yuecong

AU - Wang, Chuan

AU - Xu, Chao

AU - Yao, Qingqiang

AU - Zhao, Yuliang

AU - Chen, Chunying

AU - Wang, Yaling

PY - 2023/1/31

Y1 - 2023/1/31

N2 - Three-dimensional nondestructive, nanoresolution, and in situ visualization of protein spatial localization in a large, thick single cell remains challenging. In this study, we designed a multifunctional iron oxide (Fe@BFK) nanoprobe that possesses fluorescence and hard X-ray imaging signals. This probe can specifically target the human epidermal growth factor receptor 2 (HER2) protein and help optimize the label condition and selection of suitable samples for X-ray imaging. Combining 30 nm resolution synchrotron radiation hard X-ray nanocomputed tomography and the X-ray-sensitive Fe@BFK nanoprobe, a 3D localization of HER2 on SK-BR-3 cells was obtained for the first time. HER2 was mainly localized and cluster-distributed on the cell membrane with a heterogeneous pattern. This study provides a novel method for the in situ and nondestructive synchrotron radiation imaging of the desired protein localization in large, thick cells and evaluation of the true cellular distribution of a nanoprobe with high resolution.

AB - Three-dimensional nondestructive, nanoresolution, and in situ visualization of protein spatial localization in a large, thick single cell remains challenging. In this study, we designed a multifunctional iron oxide (Fe@BFK) nanoprobe that possesses fluorescence and hard X-ray imaging signals. This probe can specifically target the human epidermal growth factor receptor 2 (HER2) protein and help optimize the label condition and selection of suitable samples for X-ray imaging. Combining 30 nm resolution synchrotron radiation hard X-ray nanocomputed tomography and the X-ray-sensitive Fe@BFK nanoprobe, a 3D localization of HER2 on SK-BR-3 cells was obtained for the first time. HER2 was mainly localized and cluster-distributed on the cell membrane with a heterogeneous pattern. This study provides a novel method for the in situ and nondestructive synchrotron radiation imaging of the desired protein localization in large, thick cells and evaluation of the true cellular distribution of a nanoprobe with high resolution.

UR - https://www.scopus.com/pages/publications/85145462578

U2 - 10.1021/acs.analchem.2c03699

DO - 10.1021/acs.analchem.2c03699

M3 - Article

AN - SCOPUS:85145462578

SN - 0003-2700

VL - 95

SP - 2129

EP - 2133

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 4

ER -

Multifunctional Nanoprobe for 3D Nanoresolution Imaging of Intact Cell HER2 Protein with Hard X-ray Tomography

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