Microencapsulation of culture of hepatic lobule model using shape-controlled alginate-poly-L-lysine (PLL) microcapsules with the electrodeposition method

To achieve functional cell composite for tissue engineering, artificial microtissue with the shape similar to our native tissue is important to mimic and accurately recapitulate the native in vivo scenario. We proposed a novel method for fabricating hepatic lobule-like microtissue containing RLC-18 cells based on electrodeposition method. The functionality of the fabricated microtissue was compared with the cell spheroid quantitatively. A simple micro-manipulation system was established to assembly these microtissue module into 4-layerd 3D hepatic lobule-like tissue. This method provide a suitable platform to achieve 3D complex cell model with bio-function, high cell dense and architecture for the applications of artificial organ fabrication.