TY - JOUR
T1 - Microbacterium salitolerans sp. nov., isolated from saline soil
AU - Bai, Pengze
AU - Chen, Ya
AU - Liu, Fang
AU - Wang, Zhengwen
AU - Guo, Jingnan
AU - Wang, Qigang
AU - Xin, Yuhua
AU - Zhang, Jianli
PY - 2026/1/1
Y1 - 2026/1/1
N2 - Strain G2-8T was isolated from a soil sample collected from the saline area of Baodi District in Tianjin City. The isolate was a Gram-stain-positive, aerobic, non-motile, rod-shaped bacterium, and its colonies were round, raised, pale yellow, with a smooth surface, regular and clean edges. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain G2-8T belonged to the genus Microbacterium and shared the highest sequence similarity with Microbacterium excoecariae CBS5P-1T (98.5%), followed by Microbacterium amylolyticum N5T (98.2%), Microbacterium gubbeenense DSM 15944T (98.1%), Microbacterium indicum BBH6T (97.5%), Microbacterium suaedae YZYP 306T (97.5%) and Microbacterium karelineae TRM 80801T (97.4%). Phylogenomic analysis showed that strain G2-8T was related to members of the genus Microbacterium and represented an independent lineage. Growth occurred at 4-40 °C (optimum, 28 °C), up to 13.0% (w/v) NaCl (optimum, 0-2.0%) and pH 6.0-10.0 (optimum, pH 8.0). Its main fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0. The major respiratory quinone of strain G2-8T was MK-10, MK-11 and MK-12. The polar lipid profile of strain G2-8T comprised diphosphatidylglycerol, phosphatidylglycerol and two unidentified polar lipids. The genome of strain G2-8T was 3.46 Mbp with a G+C content of 69.7 mol%. The average nucleotide identity (ANI) values between strain G2-8T and other related species of the genus Microbacterium were found to be low (ANIm <84.8%, ANIb <77.2% and OrthoANIu <79.6%). Furthermore, the digital DNA-DNA hybridization and average amino acid identity values between strain G2-8T and the closely related species ranged from 19.0 to 24.2% and from 65.9 to 79.6%, respectively. Based on the results of our phylogenetic, phenotypic, genotypic and chemotaxonomic analyses, it is concluded that strain G2-8T represents a novel species of the genus Microbacterium, for which the name Microbacterium salitolerans sp. nov. is proposed. The type strain is G2-8T (=KCTC 49757T=GDMCC 1.2353T).
AB - Strain G2-8T was isolated from a soil sample collected from the saline area of Baodi District in Tianjin City. The isolate was a Gram-stain-positive, aerobic, non-motile, rod-shaped bacterium, and its colonies were round, raised, pale yellow, with a smooth surface, regular and clean edges. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain G2-8T belonged to the genus Microbacterium and shared the highest sequence similarity with Microbacterium excoecariae CBS5P-1T (98.5%), followed by Microbacterium amylolyticum N5T (98.2%), Microbacterium gubbeenense DSM 15944T (98.1%), Microbacterium indicum BBH6T (97.5%), Microbacterium suaedae YZYP 306T (97.5%) and Microbacterium karelineae TRM 80801T (97.4%). Phylogenomic analysis showed that strain G2-8T was related to members of the genus Microbacterium and represented an independent lineage. Growth occurred at 4-40 °C (optimum, 28 °C), up to 13.0% (w/v) NaCl (optimum, 0-2.0%) and pH 6.0-10.0 (optimum, pH 8.0). Its main fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0. The major respiratory quinone of strain G2-8T was MK-10, MK-11 and MK-12. The polar lipid profile of strain G2-8T comprised diphosphatidylglycerol, phosphatidylglycerol and two unidentified polar lipids. The genome of strain G2-8T was 3.46 Mbp with a G+C content of 69.7 mol%. The average nucleotide identity (ANI) values between strain G2-8T and other related species of the genus Microbacterium were found to be low (ANIm <84.8%, ANIb <77.2% and OrthoANIu <79.6%). Furthermore, the digital DNA-DNA hybridization and average amino acid identity values between strain G2-8T and the closely related species ranged from 19.0 to 24.2% and from 65.9 to 79.6%, respectively. Based on the results of our phylogenetic, phenotypic, genotypic and chemotaxonomic analyses, it is concluded that strain G2-8T represents a novel species of the genus Microbacterium, for which the name Microbacterium salitolerans sp. nov. is proposed. The type strain is G2-8T (=KCTC 49757T=GDMCC 1.2353T).
KW - 16S rRNA gene
KW - Microbacterium salitolerans sp. nov.
KW - polyphasic taxonomy
KW - whole genome
UR - https://www.scopus.com/pages/publications/105028138132
U2 - 10.1099/ijsem.0.007039
DO - 10.1099/ijsem.0.007039
M3 - Article
C2 - 41556759
AN - SCOPUS:105028138132
SN - 1466-5026
VL - 76
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 1
ER -