TY - JOUR
T1 - Microarray Based Screening of Peptide Nano Probes for HER2 Positive Tumor
AU - Wang, Zihua
AU - Wang, Weizhi
AU - Bu, Xiangli
AU - Wei, Zewen
AU - Geng, Lingling
AU - Wu, Yue
AU - Dong, Chengyan
AU - Li, Liqiang
AU - Zhang, Di
AU - Yang, Shu
AU - Wang, Fan
AU - Lausted, Christopher
AU - Hood, Leroy
AU - Hu, Zhiyuan
N1 - Publisher Copyright:
© 2015 American Chemical Society.
PY - 2015/8/18
Y1 - 2015/8/18
N2 - Peptides are excellent biointerface molecules and diagnostic probes with many advantages such as good penetration, short turnover time, and low cost. We report here an efficient peptide screening strategy based on in situ single bead sequencing on a microarray. Two novel peptides YLFFVFER (H6) and KLRLEWNR (H10) specifically binding to the tumor biomarker human epidermal growth factor receptor 2 (HER2) with aKD of 10-8 M were obtained from a 105 library. Conjugated to nanoparticles, both the H6 and H10 probes showed specific accumulation in HER2-positive tumor tissues in xenografted mice by in vivo imaging.
AB - Peptides are excellent biointerface molecules and diagnostic probes with many advantages such as good penetration, short turnover time, and low cost. We report here an efficient peptide screening strategy based on in situ single bead sequencing on a microarray. Two novel peptides YLFFVFER (H6) and KLRLEWNR (H10) specifically binding to the tumor biomarker human epidermal growth factor receptor 2 (HER2) with aKD of 10-8 M were obtained from a 105 library. Conjugated to nanoparticles, both the H6 and H10 probes showed specific accumulation in HER2-positive tumor tissues in xenografted mice by in vivo imaging.
UR - https://www.scopus.com/pages/publications/84939826094
U2 - 10.1021/acs.analchem.5b01588
DO - 10.1021/acs.analchem.5b01588
M3 - Article
C2 - 26218790
AN - SCOPUS:84939826094
SN - 0003-2700
VL - 87
SP - 8367
EP - 8372
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 16
ER -