Microarray Based Screening of Peptide Nano Probes for HER2 Positive Tumor

Zihua Wang; Weizhi Wang; Xiangli Bu; Zewen Wei; Lingling Geng; Yue Wu; Chengyan Dong; Liqiang Li; Di Zhang; Shu Yang; Fan Wang; Christopher Lausted; Leroy Hood; Zhiyuan Hu

doi:10.1021/acs.analchem.5b01588

Microarray Based Screening of Peptide Nano Probes for HER2 Positive Tumor

Zihua Wang
, Weizhi Wang^*
, Xiangli Bu
, Zewen Wei
, Lingling Geng
, Yue Wu
, Chengyan Dong
, Liqiang Li
, Di Zhang
, Shu Yang
, Fan Wang
, Christopher Lausted
, Leroy Hood
, Zhiyuan Hu

^*Corresponding author for this work

National Center for Nanoscience and Technology
Peking University
Institute for Systems Biology
Academy of Military Medical Science China

Research output: Contribution to journal › Article › peer-review

56 Citations (Scopus)

Abstract

Peptides are excellent biointerface molecules and diagnostic probes with many advantages such as good penetration, short turnover time, and low cost. We report here an efficient peptide screening strategy based on in situ single bead sequencing on a microarray. Two novel peptides YLFFVFER (H6) and KLRLEWNR (H10) specifically binding to the tumor biomarker human epidermal growth factor receptor 2 (HER2) with aK_D of 10^-8 M were obtained from a 10⁵ library. Conjugated to nanoparticles, both the H6 and H10 probes showed specific accumulation in HER2-positive tumor tissues in xenografted mice by in vivo imaging.

Original language	English
Pages (from-to)	8367-8372
Number of pages	6
Journal	Analytical Chemistry
Volume	87
Issue number	16
DOIs	https://doi.org/10.1021/acs.analchem.5b01588
Publication status	Published - 18 Aug 2015
Externally published	Yes

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title = "Microarray Based Screening of Peptide Nano Probes for HER2 Positive Tumor",

abstract = "Peptides are excellent biointerface molecules and diagnostic probes with many advantages such as good penetration, short turnover time, and low cost. We report here an efficient peptide screening strategy based on in situ single bead sequencing on a microarray. Two novel peptides YLFFVFER (H6) and KLRLEWNR (H10) specifically binding to the tumor biomarker human epidermal growth factor receptor 2 (HER2) with aKD of 10-8 M were obtained from a 105 library. Conjugated to nanoparticles, both the H6 and H10 probes showed specific accumulation in HER2-positive tumor tissues in xenografted mice by in vivo imaging.",

author = "Zihua Wang and Weizhi Wang and Xiangli Bu and Zewen Wei and Lingling Geng and Yue Wu and Chengyan Dong and Liqiang Li and Di Zhang and Shu Yang and Fan Wang and Christopher Lausted and Leroy Hood and Zhiyuan Hu",

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year = "2015",

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day = "18",

doi = "10.1021/acs.analchem.5b01588",

language = "English",

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T1 - Microarray Based Screening of Peptide Nano Probes for HER2 Positive Tumor

AU - Wang, Zihua

AU - Wang, Weizhi

AU - Bu, Xiangli

AU - Wei, Zewen

AU - Geng, Lingling

AU - Wu, Yue

AU - Dong, Chengyan

AU - Li, Liqiang

AU - Zhang, Di

AU - Yang, Shu

AU - Wang, Fan

AU - Lausted, Christopher

AU - Hood, Leroy

AU - Hu, Zhiyuan

PY - 2015/8/18

Y1 - 2015/8/18

N2 - Peptides are excellent biointerface molecules and diagnostic probes with many advantages such as good penetration, short turnover time, and low cost. We report here an efficient peptide screening strategy based on in situ single bead sequencing on a microarray. Two novel peptides YLFFVFER (H6) and KLRLEWNR (H10) specifically binding to the tumor biomarker human epidermal growth factor receptor 2 (HER2) with aKD of 10-8 M were obtained from a 105 library. Conjugated to nanoparticles, both the H6 and H10 probes showed specific accumulation in HER2-positive tumor tissues in xenografted mice by in vivo imaging.

AB - Peptides are excellent biointerface molecules and diagnostic probes with many advantages such as good penetration, short turnover time, and low cost. We report here an efficient peptide screening strategy based on in situ single bead sequencing on a microarray. Two novel peptides YLFFVFER (H6) and KLRLEWNR (H10) specifically binding to the tumor biomarker human epidermal growth factor receptor 2 (HER2) with aKD of 10-8 M were obtained from a 105 library. Conjugated to nanoparticles, both the H6 and H10 probes showed specific accumulation in HER2-positive tumor tissues in xenografted mice by in vivo imaging.

UR - https://www.scopus.com/pages/publications/84939826094

U2 - 10.1021/acs.analchem.5b01588

DO - 10.1021/acs.analchem.5b01588

M3 - Article

C2 - 26218790

AN - SCOPUS:84939826094

SN - 0003-2700

VL - 87

SP - 8367

EP - 8372

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 16

ER -

Microarray Based Screening of Peptide Nano Probes for HER2 Positive Tumor

Abstract

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