Methyl Ketones from Municipal Solid Waste Blends by One-Pot Ionic-Liquid Pretreatment, Saccharification, and Fermentation

The conversion of municipal solid waste (MSW) and lignocellulosic biomass blends to methyl ketones (MKs) was investigated by using bioderived ionic liquid (bionic liquid)-based hydrolysates followed by fermentation with an engineered Escherichia coli strain. The hydrolysates were produced by a one-pot process using six types of MSW–biomass blends, choline-based bionic liquids, and commercial enzymes. Based on the sugar yields, one blend (corn stover/MSW=95:5, w/w) and two bionic liquids {cholinium lysinate ([Ch][Lys]) and cholinium aspartate ([Ch]₂[Asp])} were selected for scale-up studies. Maximum yields of 82.3 % glucose and 54.4 % xylose were obtained from the selected blend in the scale-up studies (6 L), which was comparable with 83.6 % glucose and 52.8 % xylose obtained at a smaller scale (0.2 L). Comparable or higher yields of medium-chain (C₁₁–C₁₇) MKs were achieved by using the MSW–biomass blend-derived hydrolysates, relative to the sugar controls (glucose and xylose) with similar sugar feeding concentrations. Up to 1145 mg L⁻¹ of MKs was produced by using MSW–biomass-derived hydrolysates, and the MK titer decreased to 300 mg L⁻¹ when the bionic-liquid concentration in the hydrolysate increased from 1 to 2 %, indicative of bionic-liquid inhibition. Technoeconomic analysis was conducted to investigate the economic potential of using the selected MSW–biomass blend as a feedstock to produce MKs.