Method for suppressing 18O labeling back-exchange in off-gel fractionation

Yan Xiong; Yu Lin Deng

doi:10.15918/j.tbit1001-0645.2015.supplement1.001

Method for suppressing ¹⁸O labeling back-exchange in off-gel fractionation

Yan Xiong, Yu Lin Deng^*

^*Corresponding author for this work

School of Medical and Technology

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

Off-gel fractionation and ¹⁸O labeling are widely used in proteomics research. In this study, two methods were applied in tryptic peptides from rat striatum protein. Results show that off-gel has better separation and isoelectric focusing for complex biological samples. Furthermore, ¹⁸O labeled peptides have no apparent back-exchange, and over 90% proteins from equal amounts of ¹⁶O and ¹⁸O labeled peptides mixture have a ¹⁶O/¹⁸O ratio of 1.04±0.16 after ultrafiltration, indicating no apparent effect on quantification and ¹⁸O labeling is compatible with off-gel separation based on ultrafiltration, which can provide an alternative separation method for ¹⁸O labeling and play important role in quantitative proteomics research.

Original language	English
Pages (from-to)	1-5
Number of pages	5
Journal	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
Volume	35
DOIs	https://doi.org/10.15918/j.tbit1001-0645.2015.supplement1.001
Publication status	Published - 1 Jul 2015

Keywords

O labeling
Off-gel fractionation
Proteomics
Ultra-filtration

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10.15918/j.tbit1001-0645.2015.supplement1.001

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title = "Method for suppressing 18O labeling back-exchange in off-gel fractionation",

abstract = "Off-gel fractionation and 18O labeling are widely used in proteomics research. In this study, two methods were applied in tryptic peptides from rat striatum protein. Results show that off-gel has better separation and isoelectric focusing for complex biological samples. Furthermore, 18O labeled peptides have no apparent back-exchange, and over 90% proteins from equal amounts of 16O and 18O labeled peptides mixture have a 16O/18O ratio of 1.04±0.16 after ultrafiltration, indicating no apparent effect on quantification and 18O labeling is compatible with off-gel separation based on ultrafiltration, which can provide an alternative separation method for 18O labeling and play important role in quantitative proteomics research.",

keywords = "O labeling, Off-gel fractionation, Proteomics, Ultra-filtration",

author = "Yan Xiong and Deng, {Yu Lin}",

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doi = "10.15918/j.tbit1001-0645.2015.supplement1.001",

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AU - Xiong, Yan

AU - Deng, Yu Lin

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Off-gel fractionation and 18O labeling are widely used in proteomics research. In this study, two methods were applied in tryptic peptides from rat striatum protein. Results show that off-gel has better separation and isoelectric focusing for complex biological samples. Furthermore, 18O labeled peptides have no apparent back-exchange, and over 90% proteins from equal amounts of 16O and 18O labeled peptides mixture have a 16O/18O ratio of 1.04±0.16 after ultrafiltration, indicating no apparent effect on quantification and 18O labeling is compatible with off-gel separation based on ultrafiltration, which can provide an alternative separation method for 18O labeling and play important role in quantitative proteomics research.

AB - Off-gel fractionation and 18O labeling are widely used in proteomics research. In this study, two methods were applied in tryptic peptides from rat striatum protein. Results show that off-gel has better separation and isoelectric focusing for complex biological samples. Furthermore, 18O labeled peptides have no apparent back-exchange, and over 90% proteins from equal amounts of 16O and 18O labeled peptides mixture have a 16O/18O ratio of 1.04±0.16 after ultrafiltration, indicating no apparent effect on quantification and 18O labeling is compatible with off-gel separation based on ultrafiltration, which can provide an alternative separation method for 18O labeling and play important role in quantitative proteomics research.

KW - O labeling

KW - Off-gel fractionation

KW - Proteomics

KW - Ultra-filtration

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DO - 10.15918/j.tbit1001-0645.2015.supplement1.001

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AN - SCOPUS:84943396759

SN - 1001-0645

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JO - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

ER -

Method for suppressing ¹⁸O labeling back-exchange in off-gel fractionation

Abstract

Keywords

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