TY - JOUR
T1 - Melatonin improves the quality of inferior bovine oocytes and promoted their subsequent IVF embryo development
T2 - Mechanisms and results
AU - Yang, Minghui
AU - Tao, Jingli
AU - Chai, Menglong
AU - Wu, Hao
AU - Wang, Jing
AU - Li, Guangdong
AU - He, Changjiu
AU - Xie, Lu
AU - Ji, Pengyun
AU - Dai, Yunping
AU - Yang, Liguo
AU - Liu, Guoshi
N1 - Publisher Copyright:
© 2017 by the authors.
PY - 2017/12
Y1 - 2017/12
N2 - The inferior oocytes (IOs), which are not suitable for embryo development, occupy roughly one-third or more of the collected immature bovine oocytes. The IOs are usually discarded from the in vitro bovine embryo production process. Improving the quality of the inferior oocytes (IOs) and make them available in in vitro embryo production would have important biological, as well as commercial, value. This study was designed to investigate whether melatonin could improve the quality of IOs and make them usable in the in vitro maturation (IVM) and subsequent (in vitro fertilization) IVF embryo development. The results indicated that: the maturation rate of IOs and their subsequent IVF embryo developments were impaired compared to cumulus-oocyte complexes and melatonin treatment significantly improved the quality of IOs, as well as their IVF and embryo developments. The potential mechanisms are that: (1) melatonin reduced reactive oxygen species (ROS) and enhanced glutathione (GSH) levels in the IOs, thereby protecting them from oxidative stress; (2) melatonin improved mitochondrial normal distribution and function to increase ATP level in IOs; and (3) melatonin upregulated the expression of ATPase 6, BMP-15, GDF-9, SOD-1, Gpx-4, and Bcl-2, which are critical genes for oocyte maturation and embryo development and downregulated apoptotic gene expression of caspase-3.
AB - The inferior oocytes (IOs), which are not suitable for embryo development, occupy roughly one-third or more of the collected immature bovine oocytes. The IOs are usually discarded from the in vitro bovine embryo production process. Improving the quality of the inferior oocytes (IOs) and make them available in in vitro embryo production would have important biological, as well as commercial, value. This study was designed to investigate whether melatonin could improve the quality of IOs and make them usable in the in vitro maturation (IVM) and subsequent (in vitro fertilization) IVF embryo development. The results indicated that: the maturation rate of IOs and their subsequent IVF embryo developments were impaired compared to cumulus-oocyte complexes and melatonin treatment significantly improved the quality of IOs, as well as their IVF and embryo developments. The potential mechanisms are that: (1) melatonin reduced reactive oxygen species (ROS) and enhanced glutathione (GSH) levels in the IOs, thereby protecting them from oxidative stress; (2) melatonin improved mitochondrial normal distribution and function to increase ATP level in IOs; and (3) melatonin upregulated the expression of ATPase 6, BMP-15, GDF-9, SOD-1, Gpx-4, and Bcl-2, which are critical genes for oocyte maturation and embryo development and downregulated apoptotic gene expression of caspase-3.
KW - In vitro embryo production
KW - Inferior oocytes maturation
KW - Melatonin
KW - Mitochondria
UR - http://www.scopus.com/inward/record.url?scp=85035774135&partnerID=8YFLogxK
U2 - 10.3390/molecules22122059
DO - 10.3390/molecules22122059
M3 - Article
C2 - 29186876
AN - SCOPUS:85035774135
SN - 1420-3049
VL - 22
JO - Molecules
JF - Molecules
IS - 12
M1 - 2059
ER -