Local cell stiffness measurement using probes deformation

Masaru Takeuchi; Mohd Ridzuan Bin Ahmad; Masahiro Nakajima; Toshio Fukuda; Hiroki Umeshima; Mineko Kengaku; Yasuhisa Hasegawa; Qiang Huang

doi:10.1109/NANO.2014.6968035

Local cell stiffness measurement using probes deformation

Masaru Takeuchi^*
, Mohd Ridzuan Bin Ahmad
, Masahiro Nakajima
, Toshio Fukuda
, Hiroki Umeshima
, Mineko Kengaku
, Yasuhisa Hasegawa
, Qiang Huang

^*Corresponding author for this work

School of Mechatronical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Citations (Scopus)

Abstract

In this paper, we propose and demonstrate a new method for local cell stiffness measurement using multiple probes system. Thin glass pipettes were employed to measure the stiffness of cells on a general culture dish. The thin micropipettes were prepared and their spring constant was measured using the Atomic Force Microscopy (AFM) cantilever before stiffness measurement. The deformation of the pipette tips and the cell were observed under an optical microscope. The cell stiffness was calculated from the deformations based on the Hooke's low. The measurement results show that the myoblast C2C12 cell on a glass substrate has the stiffness around 0.1 N/m. The experimental results indicate that the proposed method can conduct the time-lapse cell stiffness measurement at multiple points simultaneously.

Original language	English
Title of host publication	Proceedings of the IEEE Conference on Nanotechnology
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	168-171
Number of pages	4
ISBN (Electronic)	9781479956227
DOIs	https://doi.org/10.1109/NANO.2014.6968035
Publication status	Published - 26 Nov 2014
Event	2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014 - Toronto, Canada Duration: 18 Aug 2014 → 21 Aug 2014

Publication series

Name	Proceedings of the IEEE Conference on Nanotechnology
ISSN (Electronic)	1944-9399

Conference

Conference	2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014
Country/Territory	Canada
City	Toronto
Period	18/08/14 → 21/08/14

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10.1109/NANO.2014.6968035

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Takeuchi, M., Bin Ahmad, M. R., Nakajima, M., Fukuda, T., Umeshima, H., Kengaku, M., Hasegawa, Y., & Huang, Q. (2014). Local cell stiffness measurement using probes deformation. In Proceedings of the IEEE Conference on Nanotechnology (pp. 168-171). Article 6968035 (Proceedings of the IEEE Conference on Nanotechnology). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NANO.2014.6968035

@inproceedings{a24b49b21a4840779d7d7b6781473f0a,

title = "Local cell stiffness measurement using probes deformation",

abstract = "In this paper, we propose and demonstrate a new method for local cell stiffness measurement using multiple probes system. Thin glass pipettes were employed to measure the stiffness of cells on a general culture dish. The thin micropipettes were prepared and their spring constant was measured using the Atomic Force Microscopy (AFM) cantilever before stiffness measurement. The deformation of the pipette tips and the cell were observed under an optical microscope. The cell stiffness was calculated from the deformations based on the Hooke's low. The measurement results show that the myoblast C2C12 cell on a glass substrate has the stiffness around 0.1 N/m. The experimental results indicate that the proposed method can conduct the time-lapse cell stiffness measurement at multiple points simultaneously.",

author = "Masaru Takeuchi and \{Bin Ahmad\}, \{Mohd Ridzuan\} and Masahiro Nakajima and Toshio Fukuda and Hiroki Umeshima and Mineko Kengaku and Yasuhisa Hasegawa and Qiang Huang",

note = "Publisher Copyright: {\textcopyright} 2014 IEEE.; 2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014 ; Conference date: 18-08-2014 Through 21-08-2014",

year = "2014",

month = nov,

day = "26",

doi = "10.1109/NANO.2014.6968035",

language = "English",

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Takeuchi, M, Bin Ahmad, MR, Nakajima, M, Fukuda, T, Umeshima, H, Kengaku, M, Hasegawa, Y & Huang, Q 2014, Local cell stiffness measurement using probes deformation. in Proceedings of the IEEE Conference on Nanotechnology., 6968035, Proceedings of the IEEE Conference on Nanotechnology, Institute of Electrical and Electronics Engineers Inc., pp. 168-171, 2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014, Toronto, Canada, 18/08/14. https://doi.org/10.1109/NANO.2014.6968035

Local cell stiffness measurement using probes deformation. / Takeuchi, Masaru; Bin Ahmad, Mohd Ridzuan; Nakajima, Masahiro et al.
Proceedings of the IEEE Conference on Nanotechnology. Institute of Electrical and Electronics Engineers Inc., 2014. p. 168-171 6968035 (Proceedings of the IEEE Conference on Nanotechnology).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Local cell stiffness measurement using probes deformation

AU - Takeuchi, Masaru

AU - Bin Ahmad, Mohd Ridzuan

AU - Nakajima, Masahiro

AU - Fukuda, Toshio

AU - Umeshima, Hiroki

AU - Kengaku, Mineko

AU - Hasegawa, Yasuhisa

AU - Huang, Qiang

PY - 2014/11/26

Y1 - 2014/11/26

N2 - In this paper, we propose and demonstrate a new method for local cell stiffness measurement using multiple probes system. Thin glass pipettes were employed to measure the stiffness of cells on a general culture dish. The thin micropipettes were prepared and their spring constant was measured using the Atomic Force Microscopy (AFM) cantilever before stiffness measurement. The deformation of the pipette tips and the cell were observed under an optical microscope. The cell stiffness was calculated from the deformations based on the Hooke's low. The measurement results show that the myoblast C2C12 cell on a glass substrate has the stiffness around 0.1 N/m. The experimental results indicate that the proposed method can conduct the time-lapse cell stiffness measurement at multiple points simultaneously.

AB - In this paper, we propose and demonstrate a new method for local cell stiffness measurement using multiple probes system. Thin glass pipettes were employed to measure the stiffness of cells on a general culture dish. The thin micropipettes were prepared and their spring constant was measured using the Atomic Force Microscopy (AFM) cantilever before stiffness measurement. The deformation of the pipette tips and the cell were observed under an optical microscope. The cell stiffness was calculated from the deformations based on the Hooke's low. The measurement results show that the myoblast C2C12 cell on a glass substrate has the stiffness around 0.1 N/m. The experimental results indicate that the proposed method can conduct the time-lapse cell stiffness measurement at multiple points simultaneously.

UR - https://www.scopus.com/pages/publications/84919472775

U2 - 10.1109/NANO.2014.6968035

DO - 10.1109/NANO.2014.6968035

M3 - Conference contribution

AN - SCOPUS:84919472775

T3 - Proceedings of the IEEE Conference on Nanotechnology

SP - 168

EP - 171

BT - Proceedings of the IEEE Conference on Nanotechnology

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014

Y2 - 18 August 2014 through 21 August 2014

ER -

Local cell stiffness measurement using probes deformation

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