Lectin chip for individally isolating of pancereatic circulating tumor cells

Taisuke Masuda; Hiroaki Tateno; Wu Lei; Izzuddin Arifin; Fumihito Arai

Lectin chip for individally isolating of pancereatic circulating tumor cells

Taisuke Masuda^*
, Hiroaki Tateno
, Wu Lei
, Izzuddin Arifin
, Fumihito Arai

^*Corresponding author for this work

Nagoya University
National Institute of Advanced Industrial Science and Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

To achieve analysis of CTCs at the single-cell level, we have applied the meniscus-induced microfluidic device, rare cell sorter. However, size-based isolation causes missing of smaller cells. In this paper, we proposed an open-channel chip that immobilized a specific lectin to assist size-based cell isolation. Immobilized lectins was used to bind a glycoprotein of pancreatic cancer cell. In this work, We demonstrated that the lectin chip with rBC2LCN provided high isolation rate and predominantly assist size-based isolation.

Original language	English
Title of host publication	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Publisher	Chemical and Biological Microsystems Society
Pages	187-188
Number of pages	2
ISBN (Electronic)	9780692941836
Publication status	Published - 2020
Externally published	Yes
Event	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States Duration: 22 Oct 2017 → 26 Oct 2017

Publication series

Name	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Country/Territory	United States
City	Savannah
Period	22/10/17 → 26/10/17

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cell Isolation
Lection
Microfluidic
Pancreatic Cancer Cell
Single-cell Recovery

Cite this

Masuda, T., Tateno, H., Lei, W., Arifin, I., & Arai, F. (2020). Lectin chip for individally isolating of pancereatic circulating tumor cells. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 (pp. 187-188). (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017). Chemical and Biological Microsystems Society.

Masuda, Taisuke ; Tateno, Hiroaki ; Lei, Wu et al. / Lectin chip for individally isolating of pancereatic circulating tumor cells. 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. Chemical and Biological Microsystems Society, 2020. pp. 187-188 (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017).

@inproceedings{6517efa1e0254355ae57795c282c5032,

title = "Lectin chip for individally isolating of pancereatic circulating tumor cells",

abstract = "To achieve analysis of CTCs at the single-cell level, we have applied the meniscus-induced microfluidic device, rare cell sorter. However, size-based isolation causes missing of smaller cells. In this paper, we proposed an open-channel chip that immobilized a specific lectin to assist size-based cell isolation. Immobilized lectins was used to bind a glycoprotein of pancreatic cancer cell. In this work, We demonstrated that the lectin chip with rBC2LCN provided high isolation rate and predominantly assist size-based isolation.",

keywords = "Cell Isolation, Lection, Microfluidic, Pancreatic Cancer Cell, Single-cell Recovery",

author = "Taisuke Masuda and Hiroaki Tateno and Wu Lei and Izzuddin Arifin and Fumihito Arai",

note = "Publisher Copyright: {\textcopyright} 17CBMS-0001.; 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 ; Conference date: 22-10-2017 Through 26-10-2017",

year = "2020",

language = "English",

series = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017",

publisher = "Chemical and Biological Microsystems Society",

pages = "187--188",

booktitle = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017",

}

Masuda, T, Tateno, H, Lei, W, Arifin, I & Arai, F 2020, Lectin chip for individally isolating of pancereatic circulating tumor cells. in 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017, Chemical and Biological Microsystems Society, pp. 187-188, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017, Savannah, United States, 22/10/17.

Lectin chip for individally isolating of pancereatic circulating tumor cells. / Masuda, Taisuke; Tateno, Hiroaki; Lei, Wu et al.
21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. Chemical and Biological Microsystems Society, 2020. p. 187-188 (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Lectin chip for individally isolating of pancereatic circulating tumor cells

AU - Masuda, Taisuke

AU - Tateno, Hiroaki

AU - Lei, Wu

AU - Arifin, Izzuddin

AU - Arai, Fumihito

N1 - Publisher Copyright: © 17CBMS-0001.

PY - 2020

Y1 - 2020

N2 - To achieve analysis of CTCs at the single-cell level, we have applied the meniscus-induced microfluidic device, rare cell sorter. However, size-based isolation causes missing of smaller cells. In this paper, we proposed an open-channel chip that immobilized a specific lectin to assist size-based cell isolation. Immobilized lectins was used to bind a glycoprotein of pancreatic cancer cell. In this work, We demonstrated that the lectin chip with rBC2LCN provided high isolation rate and predominantly assist size-based isolation.

AB - To achieve analysis of CTCs at the single-cell level, we have applied the meniscus-induced microfluidic device, rare cell sorter. However, size-based isolation causes missing of smaller cells. In this paper, we proposed an open-channel chip that immobilized a specific lectin to assist size-based cell isolation. Immobilized lectins was used to bind a glycoprotein of pancreatic cancer cell. In this work, We demonstrated that the lectin chip with rBC2LCN provided high isolation rate and predominantly assist size-based isolation.

KW - Cell Isolation

KW - Lection

KW - Microfluidic

KW - Pancreatic Cancer Cell

KW - Single-cell Recovery

UR - https://www.scopus.com/pages/publications/85079631815

M3 - Conference contribution

AN - SCOPUS:85079631815

T3 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

SP - 187

EP - 188

BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

PB - Chemical and Biological Microsystems Society

T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Y2 - 22 October 2017 through 26 October 2017

ER -

Masuda T, Tateno H, Lei W, Arifin I, Arai F. Lectin chip for individally isolating of pancereatic circulating tumor cells. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. Chemical and Biological Microsystems Society. 2020. p. 187-188. (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017).

Lectin chip for individally isolating of pancereatic circulating tumor cells

Abstract

Publication series

Conference

UN SDGs

Keywords

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Cite this