LAMP 耦合荧光侧向流层析试纸条的空间微生物快速检测技术^*

Relevant studies on the International Space Station have shown that during the long-term operation of the space station, the existence of microorganisms may pose a threat to the long-term operation of the space station and stay of astronauts. Microbial species and content monitoring are important parts of microbial control in the station. Due to resource constraints such as volume and power consumption in space applications, detection methods based on lateral flow chromatography test strips and supporting handheld devices will be more suitable for daily monitoring of space microorganisms, combined with loop-mediated isothermal amplification and up-conversion luminescence characteristic spectra. Nucleic acid identification technology can meet the needs of rapid, sensitive and specific identification of microorganisms. In this research, aiming at the potential harmful microorganisms in the space for the living environment of astronauts and instrumentation equipment, the nucleic acid identification technology based on fluorescent LAMP (Loop-mediated isothermal Amplification) of the lateral flow chromatography test strip was developed. Research results showed that this technology can achieve high sensitivity, high specificity and rapid detection of common harmful microorganisms on the space station such as Escherichia coli and Staphylococcus aureus.