Isotropic-resolution photoacoustic microscopy with multi-angle illumination

Tianxiong Wang; Naidi Sun; Ruimin Chen; Qifa Zhou; Song Hu

doi:10.1364/OL.44.000001

Isotropic-resolution photoacoustic microscopy with multi-angle illumination

Tianxiong Wang, Naidi Sun, Ruimin Chen, Qifa Zhou, Song Hu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We have developed photoacoustic microscopy (PAM) with three-dimensional (3D) micron-level spatial resolution. With multi-angle illumination, PAM images from different view angles can be simultaneously acquired for multi-view deconvolution, without the rotation of imaging targets. A side-by-side comparison of this multi-angle-illumination PAM (MAI-PAM) and conventional PAM, which share the same ultrasonic detector, was performed in phantoms and live mice. The phantom study showed that MAI-PAM achieved a high axial resolution of 3.7 μm, which was 10-fold higher than that of conventional PAM and approached the lateral resolution of 2.7 μm. Furthermore, the in vivo study demonstrated that MAI-PAM was able to image the 3D microvasculature with isotropic spatial resolution.

Original language	English
Journal	Optics Letters
Volume	44
Issue number	1
DOIs	https://doi.org/10.1364/OL.44.000001
Publication status	Published - 1 Jan 2019
Externally published	Yes

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title = "Isotropic-resolution photoacoustic microscopy with multi-angle illumination",

abstract = "We have developed photoacoustic microscopy (PAM) with three-dimensional (3D) micron-level spatial resolution. With multi-angle illumination, PAM images from different view angles can be simultaneously acquired for multi-view deconvolution, without the rotation of imaging targets. A side-by-side comparison of this multi-angle-illumination PAM (MAI-PAM) and conventional PAM, which share the same ultrasonic detector, was performed in phantoms and live mice. The phantom study showed that MAI-PAM achieved a high axial resolution of 3.7 μm, which was 10-fold higher than that of conventional PAM and approached the lateral resolution of 2.7 μm. Furthermore, the in vivo study demonstrated that MAI-PAM was able to image the 3D microvasculature with isotropic spatial resolution.",

author = "Tianxiong Wang and Naidi Sun and Ruimin Chen and Qifa Zhou and Song Hu",

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AU - Wang, Tianxiong

AU - Sun, Naidi

AU - Chen, Ruimin

AU - Zhou, Qifa

AU - Hu, Song

PY - 2019/1/1

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AB - We have developed photoacoustic microscopy (PAM) with three-dimensional (3D) micron-level spatial resolution. With multi-angle illumination, PAM images from different view angles can be simultaneously acquired for multi-view deconvolution, without the rotation of imaging targets. A side-by-side comparison of this multi-angle-illumination PAM (MAI-PAM) and conventional PAM, which share the same ultrasonic detector, was performed in phantoms and live mice. The phantom study showed that MAI-PAM achieved a high axial resolution of 3.7 μm, which was 10-fold higher than that of conventional PAM and approached the lateral resolution of 2.7 μm. Furthermore, the in vivo study demonstrated that MAI-PAM was able to image the 3D microvasculature with isotropic spatial resolution.

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Isotropic-resolution photoacoustic microscopy with multi-angle illumination

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