Isolation and structure identification of chemical constituents from Cassia occidentalis

The chemical constituent of Cassia occidentalis is studied in this work. The compounds were isolated by column chromatography on silica gel, Sephadex LH-20 and recrystallization. Their structures were determined on the basis of physico-chemical properties and spectroscopic methods such as ¹H-NMR and ¹³C-NMR. Five compounds were isolated from the chloroform fraction of 95% ethanolic extract of Cassia occidentalis seeds. They were identified as physcion (1); stigmasterol (2); 5, 7'-biphyscion (3); monopalmitin (4); stigmasterol-3-β-D-glucopyranoside (5), respectively. The last three compounds (3, 4 and 5) are isolated from the Cassia occidentalis for the first time.