Inhibition of in vitro amplification of targeted DNA fragment and activity of exonuclease I by a fullerene-oligonucleotide conjugate

Xinlin Yang; Xianmei Meng; Bo Li; Zhe Chen; Dongxu Zhao; Xin Tan; Qun Yu

doi:10.1016/j.biologicals.2007.11.006

Inhibition of in vitro amplification of targeted DNA fragment and activity of exonuclease I by a fullerene-oligonucleotide conjugate

Xinlin Yang^*, Xianmei Meng, Bo Li, Zhe Chen, Dongxu Zhao, Xin Tan, Qun Yu

^*Corresponding author for this work

School of Life Science

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Novel biological activities of a fullerene-oligonucleotide conjugate (FONC) were examined in the present report. A new FONC containing a complementary sequence to a specific region of the β-actin cDNA was synthesized. We found that the FONC at a low concentration (10^-6 M) could specifically inhibit PCR-amplification of the β-actin cDNA by inhibiting the activities of both Taq DNA polymerase and the cDNA template. This inhibition appeared to be mediated by reactive oxygen species (ROS). The FONC also exhibited the ability of antagonizing the enzymatic activity of an engineered nuclease, exonuclease I (Exo I). Our data provided some valuable evidences on the prospective use of FONC in the antisense strategy for gene silencing.

Original language	English
Pages (from-to)	223-226
Number of pages	4
Journal	Biologicals
Volume	36
Issue number	4
DOIs	https://doi.org/10.1016/j.biologicals.2007.11.006
Publication status	Published - Jul 2008

Keywords

Exonucleases
Fullerene-oligonucleotide conjugate
Polymerase chain reaction

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10.1016/j.biologicals.2007.11.006

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title = "Inhibition of in vitro amplification of targeted DNA fragment and activity of exonuclease I by a fullerene-oligonucleotide conjugate",

abstract = "Novel biological activities of a fullerene-oligonucleotide conjugate (FONC) were examined in the present report. A new FONC containing a complementary sequence to a specific region of the β-actin cDNA was synthesized. We found that the FONC at a low concentration (10-6 M) could specifically inhibit PCR-amplification of the β-actin cDNA by inhibiting the activities of both Taq DNA polymerase and the cDNA template. This inhibition appeared to be mediated by reactive oxygen species (ROS). The FONC also exhibited the ability of antagonizing the enzymatic activity of an engineered nuclease, exonuclease I (Exo I). Our data provided some valuable evidences on the prospective use of FONC in the antisense strategy for gene silencing.",

keywords = "Exonucleases, Fullerene-oligonucleotide conjugate, Polymerase chain reaction",

author = "Xinlin Yang and Xianmei Meng and Bo Li and Zhe Chen and Dongxu Zhao and Xin Tan and Qun Yu",

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language = "English",

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TY - JOUR

T1 - Inhibition of in vitro amplification of targeted DNA fragment and activity of exonuclease I by a fullerene-oligonucleotide conjugate

AU - Yang, Xinlin

AU - Meng, Xianmei

AU - Li, Bo

AU - Chen, Zhe

AU - Zhao, Dongxu

AU - Tan, Xin

AU - Yu, Qun

PY - 2008/7

Y1 - 2008/7

N2 - Novel biological activities of a fullerene-oligonucleotide conjugate (FONC) were examined in the present report. A new FONC containing a complementary sequence to a specific region of the β-actin cDNA was synthesized. We found that the FONC at a low concentration (10-6 M) could specifically inhibit PCR-amplification of the β-actin cDNA by inhibiting the activities of both Taq DNA polymerase and the cDNA template. This inhibition appeared to be mediated by reactive oxygen species (ROS). The FONC also exhibited the ability of antagonizing the enzymatic activity of an engineered nuclease, exonuclease I (Exo I). Our data provided some valuable evidences on the prospective use of FONC in the antisense strategy for gene silencing.

AB - Novel biological activities of a fullerene-oligonucleotide conjugate (FONC) were examined in the present report. A new FONC containing a complementary sequence to a specific region of the β-actin cDNA was synthesized. We found that the FONC at a low concentration (10-6 M) could specifically inhibit PCR-amplification of the β-actin cDNA by inhibiting the activities of both Taq DNA polymerase and the cDNA template. This inhibition appeared to be mediated by reactive oxygen species (ROS). The FONC also exhibited the ability of antagonizing the enzymatic activity of an engineered nuclease, exonuclease I (Exo I). Our data provided some valuable evidences on the prospective use of FONC in the antisense strategy for gene silencing.

KW - Exonucleases

KW - Fullerene-oligonucleotide conjugate

KW - Polymerase chain reaction

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U2 - 10.1016/j.biologicals.2007.11.006

DO - 10.1016/j.biologicals.2007.11.006

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AN - SCOPUS:45849131302

SN - 1045-1056

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SP - 223

EP - 226

JO - Biologicals

JF - Biologicals

IS - 4

ER -

Inhibition of in vitro amplification of targeted DNA fragment and activity of exonuclease I by a fullerene-oligonucleotide conjugate

Abstract

Keywords

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