Inhibition of cultured human retinal pigment epithelial cell by tissue-type plasminogen activator

H. Wen*, Z. Yin, Y. Wang, M. Zhu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

PURPOSE: To study the inhibition effects of human retinal pigment epithelial (RPE) cells by tissue-type plasminogen activator(tPA). METHODS: The effects of tPA on human RPE cells proliferation were studied by viable cell counting and methyl thiazolyl tetrazolium (MTT) colorimetric assay. Flow cytometry (FCM) analysis was used to examine RPE cell cycles. RESULTS: The proliferation of human RPE cells could be inhibited by 0.1-3 micrograms/ml tPA (P < 0.05). The effects of inhibition were depended on the drug density and the action time directly (P < 0.01). 5 micrograms/ml tPA was deadly-toxic to human RPE cells. FCM revealed that the cells in S phase increased 9.8% (P < 0.05) and cells in G2M phase decreased 6.6% (P < 0.05), compared to controls. CONCLUSION: tPA at a certain dose range and period could inhibit human RPE cells proliferation without deadly-toxicity. The human RPE cells growth could be interfered in S phase by tPA.

Original languageEnglish
Pages (from-to)122-125
Number of pages4
JournalYan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu
Volume17
Issue number2
Publication statusPublished - Jun 2001
Externally publishedYes

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