TY - GEN
T1 - Incorporation of 3-hydroxyphenylalanine into proteins using aaRS/tRNA pair
AU - Zhu, Hanrong
AU - Ma, Xiaoyan
AU - Huo, Yixin
N1 - Publisher Copyright:
© 2024 Copyright held by the owner/author(s).
PY - 2024/12/2
Y1 - 2024/12/2
N2 - Incorporation of non-canonical amino acids (ncAAs) into proteins has emerged as a powerful approach to modify the properties of natural proteins, leading to enhanced and expanded protein functions. Aromatic ncAAs represent a significant class of ncAAs that have been employed for engineering enzyme catalytic efficiency, protein structure studies, and the development of biomaterials. This study focused on the incorporation of 3-hydroxyphenylalanine (3HLP), a non-proteinogenic aromatic amino acid, and aims to establish a 3HLP incorporation system. We constructed an incorporation detection system based on the sfGFP fluorescent protein and screened out the chPheRS2/tRNACUA pair for 3HLP incorporation, expanding the application of aromatic ncAAs in protein engineering. In addition, molecular docking was analyzed by computer simulation.
AB - Incorporation of non-canonical amino acids (ncAAs) into proteins has emerged as a powerful approach to modify the properties of natural proteins, leading to enhanced and expanded protein functions. Aromatic ncAAs represent a significant class of ncAAs that have been employed for engineering enzyme catalytic efficiency, protein structure studies, and the development of biomaterials. This study focused on the incorporation of 3-hydroxyphenylalanine (3HLP), a non-proteinogenic aromatic amino acid, and aims to establish a 3HLP incorporation system. We constructed an incorporation detection system based on the sfGFP fluorescent protein and screened out the chPheRS2/tRNACUA pair for 3HLP incorporation, expanding the application of aromatic ncAAs in protein engineering. In addition, molecular docking was analyzed by computer simulation.
KW - 3-hydroxyphenylalanine
KW - Molecular docking
KW - Non-canonical amino acids
KW - Orthogonal translation system
UR - http://www.scopus.com/inward/record.url?scp=85216114110&partnerID=8YFLogxK
U2 - 10.1145/3700486.3700489
DO - 10.1145/3700486.3700489
M3 - Conference contribution
AN - SCOPUS:85216114110
T3 - ACM International Conference Proceeding Series
SP - 15
EP - 19
BT - Proceedings of 2024 International Conference on Biomedicine and Intelligent Technology, ICBIT 2024
PB - Association for Computing Machinery
T2 - 2024 International Conference on Biomedicine and Intelligent Technology, ICBIT 2024
Y2 - 23 August 2024 through 25 August 2024
ER -