Immuno-effect of plasmacytoid dendritic cells on bacteria infection induced spontaneous remission of leukemia

Lijuan Li; Liansheng Zhang; Ye Chai; Pengyun Zeng; Chongyang Wu; Lingling Yue; Jun Bai; Zhengdong Hao; Wanli Hu; Huiling Chen; Xiaojia Guo

doi:10.3760/cma.j.issn.0253-2727.2014.10.002

Immuno-effect of plasmacytoid dendritic cells on bacteria infection induced spontaneous remission of leukemia

Lijuan Li
, Liansheng Zhang
, Ye Chai
, Pengyun Zeng
, Chongyang Wu
, Lingling Yue
, Jun Bai
, Zhengdong Hao
, Wanli Hu
, Huiling Chen
, Xiaojia Guo

Lanzhou University

Research output: Contribution to journal › Article › peer-review

Abstract

OBJECTIVE: To explore the immuno-effect of plasmacytoid dendritic cells (pDC) on bacteria infection induced spontaneous remission (SR) of leukemia.

METHODS: Both pDC and myeloid dendritic cells (mDC) were isolated and purified from leukemic patient with SR and healthy donor by combination of immunomagnetic beads and flow cytometry. pDC were cultured in RPMI1640 medium and stimulated with different bacteria. The T cells proliferation was detected by MTT, and cytokine production by ELISA kits.

RESULTS: The human bacterial pathogen Staphylococcus aureus and Pseudomonas aeruginosa stimulation for 48 h resulted in the maturation of pDC with production of high quantity of IFN-α at (15.34 ± 2.91) ng/ml and (10.38 ± 1.41) ng/ml, respectively, comparing with that of negative group at (1.36 ± 0.13) ng/ml (P<0.01). Activated pDC could promote the differentiation of naive CD4⁺ T cells to Th1 cells with secretion of IFN-γ at (2.16 ± 0.37) ng/ml and (2.73 ± 1.11) ng/ml, respectively, comparing with that of positive control at (2.55 ± 0.23) ng/ml (P > 0.05). Activated pDC showed higher T cell stimulatory capacities [proliferation index (PI) was 4.36 and 4.05, respectively] than that of non-activated pDC (PI was 1.23 and 0.13, respectively) (P < 0.01).

CONCLUSION: Staphylococcus aureus and Pseudomonas aeruginosa activated pDC may play a key role in SR of leukemia following severe infections.

Original language	English
Pages (from-to)	880-884
Number of pages	5
Journal	Chinese Journal of Hematology
Volume	35
Issue number	10
DOIs	https://doi.org/10.3760/cma.j.issn.0253-2727.2014.10.002
Publication status	Published - 1 Oct 2014
Externally published	Yes

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SDG 3 Good Health and Well-being

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10.3760/cma.j.issn.0253-2727.2014.10.002

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title = "Immuno-effect of plasmacytoid dendritic cells on bacteria infection induced spontaneous remission of leukemia",

abstract = "OBJECTIVE: To explore the immuno-effect of plasmacytoid dendritic cells (pDC) on bacteria infection induced spontaneous remission (SR) of leukemia.METHODS: Both pDC and myeloid dendritic cells (mDC) were isolated and purified from leukemic patient with SR and healthy donor by combination of immunomagnetic beads and flow cytometry. pDC were cultured in RPMI1640 medium and stimulated with different bacteria. The T cells proliferation was detected by MTT, and cytokine production by ELISA kits.RESULTS: The human bacterial pathogen Staphylococcus aureus and Pseudomonas aeruginosa stimulation for 48 h resulted in the maturation of pDC with production of high quantity of IFN-α at (15.34 ± 2.91) ng/ml and (10.38 ± 1.41) ng/ml, respectively, comparing with that of negative group at (1.36 ± 0.13) ng/ml (P<0.01). Activated pDC could promote the differentiation of naive CD4⁺ T cells to Th1 cells with secretion of IFN-γ at (2.16 ± 0.37) ng/ml and (2.73 ± 1.11) ng/ml, respectively, comparing with that of positive control at (2.55 ± 0.23) ng/ml (P > 0.05). Activated pDC showed higher T cell stimulatory capacities [proliferation index (PI) was 4.36 and 4.05, respectively] than that of non-activated pDC (PI was 1.23 and 0.13, respectively) (P < 0.01).CONCLUSION: Staphylococcus aureus and Pseudomonas aeruginosa activated pDC may play a key role in SR of leukemia following severe infections.",

author = "Lijuan Li and Liansheng Zhang and Ye Chai and Pengyun Zeng and Chongyang Wu and Lingling Yue and Jun Bai and Zhengdong Hao and Wanli Hu and Huiling Chen and Xiaojia Guo",

year = "2014",

month = oct,

day = "1",

doi = "10.3760/cma.j.issn.0253-2727.2014.10.002",

language = "English",

volume = "35",

pages = "880--884",

journal = "Chinese Journal of Hematology",

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}

TY - JOUR

T1 - Immuno-effect of plasmacytoid dendritic cells on bacteria infection induced spontaneous remission of leukemia

AU - Li, Lijuan

AU - Zhang, Liansheng

AU - Chai, Ye

AU - Zeng, Pengyun

AU - Wu, Chongyang

AU - Yue, Lingling

AU - Bai, Jun

AU - Hao, Zhengdong

AU - Hu, Wanli

AU - Chen, Huiling

AU - Guo, Xiaojia

PY - 2014/10/1

Y1 - 2014/10/1

N2 - OBJECTIVE: To explore the immuno-effect of plasmacytoid dendritic cells (pDC) on bacteria infection induced spontaneous remission (SR) of leukemia.METHODS: Both pDC and myeloid dendritic cells (mDC) were isolated and purified from leukemic patient with SR and healthy donor by combination of immunomagnetic beads and flow cytometry. pDC were cultured in RPMI1640 medium and stimulated with different bacteria. The T cells proliferation was detected by MTT, and cytokine production by ELISA kits.RESULTS: The human bacterial pathogen Staphylococcus aureus and Pseudomonas aeruginosa stimulation for 48 h resulted in the maturation of pDC with production of high quantity of IFN-α at (15.34 ± 2.91) ng/ml and (10.38 ± 1.41) ng/ml, respectively, comparing with that of negative group at (1.36 ± 0.13) ng/ml (P<0.01). Activated pDC could promote the differentiation of naive CD4⁺ T cells to Th1 cells with secretion of IFN-γ at (2.16 ± 0.37) ng/ml and (2.73 ± 1.11) ng/ml, respectively, comparing with that of positive control at (2.55 ± 0.23) ng/ml (P > 0.05). Activated pDC showed higher T cell stimulatory capacities [proliferation index (PI) was 4.36 and 4.05, respectively] than that of non-activated pDC (PI was 1.23 and 0.13, respectively) (P < 0.01).CONCLUSION: Staphylococcus aureus and Pseudomonas aeruginosa activated pDC may play a key role in SR of leukemia following severe infections.

AB - OBJECTIVE: To explore the immuno-effect of plasmacytoid dendritic cells (pDC) on bacteria infection induced spontaneous remission (SR) of leukemia.METHODS: Both pDC and myeloid dendritic cells (mDC) were isolated and purified from leukemic patient with SR and healthy donor by combination of immunomagnetic beads and flow cytometry. pDC were cultured in RPMI1640 medium and stimulated with different bacteria. The T cells proliferation was detected by MTT, and cytokine production by ELISA kits.RESULTS: The human bacterial pathogen Staphylococcus aureus and Pseudomonas aeruginosa stimulation for 48 h resulted in the maturation of pDC with production of high quantity of IFN-α at (15.34 ± 2.91) ng/ml and (10.38 ± 1.41) ng/ml, respectively, comparing with that of negative group at (1.36 ± 0.13) ng/ml (P<0.01). Activated pDC could promote the differentiation of naive CD4⁺ T cells to Th1 cells with secretion of IFN-γ at (2.16 ± 0.37) ng/ml and (2.73 ± 1.11) ng/ml, respectively, comparing with that of positive control at (2.55 ± 0.23) ng/ml (P > 0.05). Activated pDC showed higher T cell stimulatory capacities [proliferation index (PI) was 4.36 and 4.05, respectively] than that of non-activated pDC (PI was 1.23 and 0.13, respectively) (P < 0.01).CONCLUSION: Staphylococcus aureus and Pseudomonas aeruginosa activated pDC may play a key role in SR of leukemia following severe infections.

UR - https://www.scopus.com/pages/publications/84929512690

U2 - 10.3760/cma.j.issn.0253-2727.2014.10.002

DO - 10.3760/cma.j.issn.0253-2727.2014.10.002

M3 - Article

C2 - 25339322

AN - SCOPUS:84929512690

SN - 0253-2727

VL - 35

SP - 880

EP - 884

JO - Chinese Journal of Hematology

JF - Chinese Journal of Hematology

IS - 10

ER -

Immuno-effect of plasmacytoid dendritic cells on bacteria infection induced spontaneous remission of leukemia

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