Immobilization of formate dehydrogenase on polyethyleneimine modified carriers for the enhancement of catalytic performance

Meng Ya Guo; Ting Ting Zhai; Cai Hong Wang; Zi Hui Meng; Wen Fang Liu

doi:10.1016/j.catcom.2020.106259

Immobilization of formate dehydrogenase on polyethyleneimine modified carriers for the enhancement of catalytic performance

Meng Ya Guo, Ting Ting Zhai, Cai Hong Wang, Zi Hui Meng, Wen Fang Liu^*

^*Corresponding author for this work

School of Chemistry and Chemical Engineering

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Polyethyleneimine (PEI) is a common modifier in the field of CO₂ adsorption but rarely used in the immobilization of enzyme catalyzing CO₂ conversion. Here, formate dehydrogenase (FDH) was immobilized on the surface of PEI modified polyethylene membrane or silica microspheres and then applied in the reaction of CO₂ to formate. After optimizing the immobilization conditions, the catalytic performance of immobilized FDH was respectively 4.3 and 7.4 times of free enzyme attributed to the promotion effect of the carrier. Polydopamine/PEI-SiO₂ attached FDH exhibited a higher activity and stability than biocatalytic membrane.

Original language	English
Article number	106259
Journal	Catalysis Communications
Volume	149
DOIs	https://doi.org/10.1016/j.catcom.2020.106259
Publication status	Published - 15 Jan 2021

Keywords

CO conversion
Enzyme immobilization
Formate dehydrogenase
Polyethylenimine
Synergistic effect

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10.1016/j.catcom.2020.106259

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title = "Immobilization of formate dehydrogenase on polyethyleneimine modified carriers for the enhancement of catalytic performance",

abstract = "Polyethyleneimine (PEI) is a common modifier in the field of CO2 adsorption but rarely used in the immobilization of enzyme catalyzing CO2 conversion. Here, formate dehydrogenase (FDH) was immobilized on the surface of PEI modified polyethylene membrane or silica microspheres and then applied in the reaction of CO2 to formate. After optimizing the immobilization conditions, the catalytic performance of immobilized FDH was respectively 4.3 and 7.4 times of free enzyme attributed to the promotion effect of the carrier. Polydopamine/PEI-SiO2 attached FDH exhibited a higher activity and stability than biocatalytic membrane.",

keywords = "CO conversion, Enzyme immobilization, Formate dehydrogenase, Polyethylenimine, Synergistic effect",

author = "Guo, {Meng Ya} and Zhai, {Ting Ting} and Wang, {Cai Hong} and Meng, {Zi Hui} and Liu, {Wen Fang}",

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doi = "10.1016/j.catcom.2020.106259",

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T1 - Immobilization of formate dehydrogenase on polyethyleneimine modified carriers for the enhancement of catalytic performance

AU - Guo, Meng Ya

AU - Zhai, Ting Ting

AU - Wang, Cai Hong

AU - Meng, Zi Hui

AU - Liu, Wen Fang

PY - 2021/1/15

Y1 - 2021/1/15

N2 - Polyethyleneimine (PEI) is a common modifier in the field of CO2 adsorption but rarely used in the immobilization of enzyme catalyzing CO2 conversion. Here, formate dehydrogenase (FDH) was immobilized on the surface of PEI modified polyethylene membrane or silica microspheres and then applied in the reaction of CO2 to formate. After optimizing the immobilization conditions, the catalytic performance of immobilized FDH was respectively 4.3 and 7.4 times of free enzyme attributed to the promotion effect of the carrier. Polydopamine/PEI-SiO2 attached FDH exhibited a higher activity and stability than biocatalytic membrane.

AB - Polyethyleneimine (PEI) is a common modifier in the field of CO2 adsorption but rarely used in the immobilization of enzyme catalyzing CO2 conversion. Here, formate dehydrogenase (FDH) was immobilized on the surface of PEI modified polyethylene membrane or silica microspheres and then applied in the reaction of CO2 to formate. After optimizing the immobilization conditions, the catalytic performance of immobilized FDH was respectively 4.3 and 7.4 times of free enzyme attributed to the promotion effect of the carrier. Polydopamine/PEI-SiO2 attached FDH exhibited a higher activity and stability than biocatalytic membrane.

KW - CO conversion

KW - Enzyme immobilization

KW - Formate dehydrogenase

KW - Polyethylenimine

KW - Synergistic effect

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DO - 10.1016/j.catcom.2020.106259

M3 - Article

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SN - 1566-7367

VL - 149

JO - Catalysis Communications

JF - Catalysis Communications

M1 - 106259

ER -

Immobilization of formate dehydrogenase on polyethyleneimine modified carriers for the enhancement of catalytic performance

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Keywords

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