TY - JOUR
T1 - Identification, classification and expression analysis of the Ras superfamily genes in the Pacific white shrimp, Litopenaeus vannamei
AU - Si, Shuqing
AU - Zhang, Xiaojun
AU - Yuan, Jianbo
AU - Zhang, Xiaoxi
AU - Yu, Yang
AU - Yang, Song
AU - Li, Fuhua
N1 - Publisher Copyright:
Copyright © 2023 Si, Zhang, Yuan, Zhang, Yu, Yang and Li.
PY - 2023/1/23
Y1 - 2023/1/23
N2 - The Ras superfamily of small guanosine triphosphatases (GTPases) are a large group of small GTP-binding proteins, which play crucial roles in basic cellular processes in all eukaryotes. In this study, by analyzing the gene structure, temporal and spatial expression patterns, a total of 108 Ras superfamily genes were identified in the genome of the Pacific white shrimp Litopenaeus vannamei. We found these genes included not only the classical Ras GTPase superfamily members, but also some unconventional and novel Ras GTPase proteins, which have unknown functions and unique expression patterns. All Ras superfamily genes of L. vannamei were highly conserved within the core G domain and closely related in phylogeny, but they might have two different evolutionary origins. In addition, different Ras GTPase genes exhibited distinct expression patterns in different tissues, development/molting stages and WSSV infection samples of L. vannamei, suggesting that they may have a high functional specialization, and play important roles in regulating the biological processes of cell differentiation, growth and development, immune response, etc. This study provides important clues for the structure, classification, evolution and function of Ras superfamily in shrimp.
AB - The Ras superfamily of small guanosine triphosphatases (GTPases) are a large group of small GTP-binding proteins, which play crucial roles in basic cellular processes in all eukaryotes. In this study, by analyzing the gene structure, temporal and spatial expression patterns, a total of 108 Ras superfamily genes were identified in the genome of the Pacific white shrimp Litopenaeus vannamei. We found these genes included not only the classical Ras GTPase superfamily members, but also some unconventional and novel Ras GTPase proteins, which have unknown functions and unique expression patterns. All Ras superfamily genes of L. vannamei were highly conserved within the core G domain and closely related in phylogeny, but they might have two different evolutionary origins. In addition, different Ras GTPase genes exhibited distinct expression patterns in different tissues, development/molting stages and WSSV infection samples of L. vannamei, suggesting that they may have a high functional specialization, and play important roles in regulating the biological processes of cell differentiation, growth and development, immune response, etc. This study provides important clues for the structure, classification, evolution and function of Ras superfamily in shrimp.
KW - gene expression
KW - gene structure and function
KW - growth
KW - GTPase proteins
KW - immunity
KW - Litopenaeus vannamei
KW - Ras superfamily
UR - http://www.scopus.com/inward/record.url?scp=85147452614&partnerID=8YFLogxK
U2 - 10.3389/fmars.2023.1063857
DO - 10.3389/fmars.2023.1063857
M3 - Article
AN - SCOPUS:85147452614
SN - 2296-7745
VL - 10
JO - Frontiers in Marine Science
JF - Frontiers in Marine Science
M1 - 1063857
ER -