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High-throughput peptide screening on a bimodal imprinting chip through ms-spri integration

  • Weizhi Wang
  • , Qiaojun Fang
  • , Zhiyuan Hu

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Screening of high affinity and high specificity peptide probes towards various targets is important in the biomedical field while traditional peptide screening procedure is manual and tedious. Herein, a bimodal imprinting microarray system to embrace the whole peptide screening process is presented. Surface Plasmon Resonance imaging (SPRi) and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) are combined for both quantitative and qualitative identification of the peptide. The method provides a solution for high efficiency peptide screening.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages111-125
Number of pages15
DOIs
Publication statusPublished - 2016
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1352
ISSN (Print)1064-3745

Keywords

  • Bimodal imprinting chip
  • MALDI-TOF-MS
  • Microarray
  • OBOC
  • SPRi

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