High-throughput peptide screening on a bimodal imprinting chip through ms-spri integration

Weizhi Wang, Qiaojun Fang, Zhiyuan Hu

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Citations (Scopus)

Abstract

Screening of high affinity and high specificity peptide probes towards various targets is important in the biomedical field while traditional peptide screening procedure is manual and tedious. Herein, a bimodal imprinting microarray system to embrace the whole peptide screening process is presented. Surface Plasmon Resonance imaging (SPRi) and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) are combined for both quantitative and qualitative identification of the peptide. The method provides a solution for high efficiency peptide screening.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages111-125
Number of pages15
DOIs
Publication statusPublished - 2016
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1352
ISSN (Print)1064-3745

Keywords

  • Bimodal imprinting chip
  • MALDI-TOF-MS
  • Microarray
  • OBOC
  • SPRi

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