TY - GEN
T1 - High-speed autofocusing of multisized microobjects
AU - Nguyen, Chanh Nghiem
AU - Ohara, Kenichi
AU - Takubo, Tomohito
AU - Mae, Yasushi
AU - Arai, Tatsuo
PY - 2012
Y1 - 2012
N2 - This paper proposes a new high-speed autofocus algorithm for observing multisized microobjects under a transmitted light microscope. The method estimates the blurriness of the object by analyzing the intensity variation of a defined region around the object border in the frequency domain. A defocus function is proposed so that the best focused position of the object is found when the defocus function reaches its minimum within the close region around the focal plane. Experiments for execution time and accuracy evaluation have been performed to compare its performance to other autofocus algorithms. Experimental results showed that the proposed algorithm outperformed other algorithms in terms of execution time and accuracy and that it is more robust to environmental change.
AB - This paper proposes a new high-speed autofocus algorithm for observing multisized microobjects under a transmitted light microscope. The method estimates the blurriness of the object by analyzing the intensity variation of a defined region around the object border in the frequency domain. A defocus function is proposed so that the best focused position of the object is found when the defocus function reaches its minimum within the close region around the focal plane. Experiments for execution time and accuracy evaluation have been performed to compare its performance to other autofocus algorithms. Experimental results showed that the proposed algorithm outperformed other algorithms in terms of execution time and accuracy and that it is more robust to environmental change.
UR - https://www.scopus.com/pages/publications/84872587917
U2 - 10.1109/CoASE.2012.6386495
DO - 10.1109/CoASE.2012.6386495
M3 - Conference contribution
AN - SCOPUS:84872587917
SN - 9781467304283
T3 - IEEE International Conference on Automation Science and Engineering
SP - 34
EP - 39
BT - 2012 IEEE International Conference on Automation Science and Engineering
T2 - 2012 IEEE International Conference on Automation Science and Engineering: Green Automation Toward a Sustainable Society, CASE 2012
Y2 - 20 August 2012 through 24 August 2012
ER -